罗有文，吴海涛，曹斌，卓国荣，吴锦

摘要：本试验旨在研究Zn-Pal对犬肠道菌群和血清免疫、抗氧化能力的影响。将20只9月龄比格犬随机分成4组，每组5只，分别饲喂基础日粮+ZnSO4（锌添加水平为80 mg/kg，对照组）、基础日粮+2.55 g/kg Zn-Pal（锌添加水平为60 mg/kg，Zn-Pal-60）、基础日粮+3.42 g/kg Zn-Pal（锌添加水平为80 mg/kg，Zn-Pal-80）、基础日粮+4.30 g/kg Zn-Pal（锌添加水平为100 mg/kg，Zn-Pal-100）。30 d后对各组犬只肠道主要菌群和血清免疫、抗氧化指标进行测定。结果显示，Zn-Pal-80组和Zn-Pal-100组大肠杆菌数量显著低于对照组（P<0.05），血清IL-6含量显著高于对照组（P<0.05）;Zn-Pal-100组血清IgG含量显著高于对照组（P<0.05）;Zn-Pal-80组血清SOD活性显著高于对照组（P<0.05），Zn-Pal-100组血清MDA含量显著低于对照组（P<0.05）。由此可见，Zn-Pal可以降低比格犬大肠杆菌数量，改善肠道微生态环境，提高比格犬血清免疫和抗氧化能力。

关键词：锌;凹凸棒石黏土;比格犬;肠道菌群;血清免疫;抗氧化

中图分类号：S829.2文献标识码：A文章编号：1000-4440（2021）06-1488-05

Effects of zinc-bearing palygorskite on intestinal microflora and antioxidant capability in dogs

LUO You-wen，WU Hai-tao，CAO Bin，ZHUO Guo-rong，WU Jin

（Jiangsu Agri-animal Husbandry Vocational College， Taizhou 225300，China）

Abstract：The study was conducted to explore the the effects of Zn-Pal on intestinal microflora and serum immunity， antioxidant capability in dogs.Twenty nine-month-old beagle dogs were randomly divided into four groups with five dogs in each group. They were fed with the basal diet +ZnSO4 （zinc supplemental level was 80mg/kg， control group）， basal diet +2.55 g/kg Zn-Pal （zinc supplemental level was 60mg/kg， Zn-Pal-60）， basal diet +3.42 g/kg Zn-Pal （zinc supplemental level was 80mg/kg， Zn-Pal-80）， and basal diet +4.30 g/kg Zn-Pal （zinc supplemental level was 100mg/kg， Zn-Pal-100）， respectively. After 30 days， the main serum immunity， antioxidant indices and intestinal microflora of beagle dogs in each group were determined. The results showed that the number of Escherichia coli in groups Zn-Pal-80 and Zn-Pal-100 was significantly lower than that in control group （P<0.05）， the serum IL-6 content in groups Zn-Pal-80 and Zn-Pal-100 was significantly higher than that in control group （P<0.05）， and the serum IgG content in group Zn-Pal-100 was significantly higher than that in control group （P<0.05）. The serum SOD activity of group Zn-Pal-80 was significantly higher than that in control group （P<0.05）. The serum MDA content of group Zn-Pal-100 was significantly lower than that in control group （P<0.05）. In conclusion， Zn-Pal can improve the intestinal environment， reduce the number of E.coli and improve the serum immunity and antioxidant capacity of beagle dogs.

Key words：zinc;palygorskite;beagle dog;intestinal microflora;serum immunity;antioxidant

鋅是犬的6种必需微量矿物元素之一，对犬的正常生理代谢起着重要的作用[1]。在动物体内锌有许多功能，它是200多种含锌酶的辅助因子和催化剂，并参与细胞分裂、有机物代谢等，也是各种蛋白质的主要成分（生长转录因子、受体、细胞因子、酶），是它们发挥生物活性所必需的物质[1-3]。锌也是宠物生长、抗氧化系统和正常免疫功能所必需的物质[4]，此外，研究结果表明，锌离子还具有一定的抗菌作用[5-6]。锌在宠物饲料中来源各异，不同形式的锌添加到日粮中均能改善动物肠道菌群[4、7-8]。抗生素在饲料中的普遍应用极大地推动了畜牧业的发展，但其带来的负面影响（抗生素残留、免疫抑制、细菌耐药性等）也逐渐显露出来。……