黄博闻，崔凯，余向阳，葛静

摘要：为揭示噻虫嗪对水稻内生细菌群落的影响，采用Illumina HiSeq高通量测序技术对室内盆栽的水稻根、茎、叶内生细菌进行16S rRNA测序。获得用于分析的686 889条优质序列和265个操作分类单元（OTU）。通过α多样性分析发现，土壤噻虫嗪处理对根中内生细菌群落多样性影响显著，对茎、叶影响不明显。在属水平上对代表性OTU进行注释，发现水稻各器官中内生细菌组成受噻虫嗪的影响明显，表明噻虫嗪对水稻内生细菌组成结构影响显著。水稻各器官中内生细菌群落组成与噻虫嗪残留浓度有一定相关性，在水稻茎、叶中，相比于低施用量噻虫嗪处理，内生菌群落在高施用量噻虫嗪处理下改变更明显。

关键词：新烟碱杀虫剂;噻虫嗪胁迫;水稻内生细菌;高通量测序

中图分类号：X592文献标识码：A文章编号：1000-4440（2021）06-1370-08

Effects of thiamethoxam stress on the community composition and diversity of endophytic bacteria in rice

HUANG Bo-wen1，CUI Kai2，YU Xiang-yang1，2，GE Jing1，2

（1.School of Environmental and Safety Engineering， Jiangsu University， Zhenjiang 212013， China;2.Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base， Ministry of Science and Technology， Nanjing 210014， China）

Abstract： The effects of thiamethoxam stress on endophytic bacterial communities in roots， stems and leaves of rice were studied by Illumina Hiseq high-throughput sequencing technology with indoor pot culture and soil addition treatment. High quality sequences and operational taxons （OTUs） were 686 889 and 265， respectively. The results of α diversity index showed that thiamethoxam had a significant effect on the structure and diversity of endophytic bacterial community in rice roots， but had no significant effect on the diversity of endophytic bacterial community in rice stems and leaves. The representative OTUs were annotated at the genus level. It was found that the composition of endophytic bacteria in rice organs was significantly affected by thiamethoxam， and which was in correlation with the residual level of thiamethoxam. The changes of endophytic bacterial community under the treatment with high application rate of thiamethoxam were more obvious than those under the treatment with low application rate of thiamethoxam in the stems and leaves of rice.

Key words：neonicotinoids;thiamethoxam stress;rice endophytic bacteria;high-throughput sequencing

內生细菌指可以在植物内部组织中定殖的，不会造成任何直接的、明显的负面影响的细菌[1]。内生细菌具有修复污染、促生抗病[2]等功效，对内生细菌合理使用能够实现农业中减肥、减药、修复土壤环境的目的。内生细菌存在于植物的各个部分，目前已发现其存在于植物的根、茎、叶、胚、种子等器官组织的细胞或细胞间隙中，在植物的根瘤中也存在内生细菌[3]。内生细菌群落分布与组成易受环境、温度、土壤类型等因素影响而产生差异[4]。Hameed等[5]发现外源污染物在水稻体内累积后会影响其器官中内生菌群落组成。Ge等[6]研究发现，土壤中塑化剂在青菜体内富集后显著改变了其原有内生菌群落组成。……