李浩然 张树伟 王金英 黄小雄 彭宏祥 徐炯志 潘介春 朱建华 谭春露 李平 丁峰

摘  要：以龙眼成熟叶片为材料，克隆龙眼MYB-related基因家族TRF2-like基因，命名为DlTRF2-like，并对其进行生物信息学和表达模式分析。DlTRF2-like基因属于MYB-related家族的TRF-like亚家族，ORF长度是909 bp，编码含有302个氨基酸残基的蛋白，预测该基因定位于细胞核，同源基因进化分析表明龙眼DlTRF2-like与阿月浑子PvTRF2-like亲缘关系最近。利用实时荧光定量PCR检测DlTRF2-like基因在‘四季蜜’龙眼不同组织及乙烯利和多效唑处理后不同时间的相对表达量。结果表明：DlTRF2-like在龙眼不同组织中均有表达，在花芽中表达量最高;乙烯利和多效唑处理后，DlTRF2-like基因的表达量明显高于对照，推测DlTRF2-like响应乙烯利和多效唑信号促进龙眼成花。

关键词：龙眼;MYB-related;龙眼成花;TRF-like亚家族;表达模式分析

中图分类号：S667.2      文献标识码：A

Abstract： The TRF2-like gene of MYB-related family was cloned from the mature leaves of longan and named DLTRF2-like. Bioinformatics and the expression pattern of DLTRF2-like were analyzed. DLTRF2-like gene belongs to the TRF-like subfamily of MYB-related family， with an ORF length of 909 bp， encoding a protein of 302 amino acids which was predicted to be located in the nucleus. Evolution analysis of homologous genes indicated that DLTRF2-like was most closely related to PvTRF2-like in Pistacia vera L. The expression pattern of DLTRF2-like in different tissues and leaves treated by ethephon and paclobutrazol was analyzed by real-time quantitative PCR. The results showed that DLTRF2-like was expressed in all the samples， and the highest expression level was found in flower buds. The expression level of DLTRF2-like gene in the leaves treated by ethephon and paclobutrazol was significantly higher than that of the control. The results indicated that DLTRF2-like might response to ethephon and paclobutrazol signal to promote the flowering of longan.

Keywords： longan; MYB-related; longan flowering; TRF-like subfamily; expression pattern analysis

DOI： 10.3969/j.issn.1000-2561.2021.12.012

龍眼（Dimocarpus longan Lour.）是无患子科龙眼属常绿果树，在我国栽培历史悠久。广西是龙眼主产地之一，采收期在7月中下旬至8月下旬。产期过于集中是我国龙眼生产面临的主要问题之一，为了有效调节龙眼产期，提高龙眼生产效益，生产上多采用修剪和化学调控等方法促进龙眼成花。化学调控主要采用植物生长调节剂和氯酸钾诱导龙眼成花，但是不同品种及不同地区的调控效果存在差异。‘四季蜜’可一年多次成花，常出现花果同期现象，生产上常用乙烯利、多效唑等处理，调控‘四季蜜’龙眼成花坐果时间。因此，解析龙眼成花的分子调控机制具有重要的理论和生产意义。

MYB转录因子参与调控植物的生长发育、生长代谢和细胞形态建成等生物学过程，在植物响应逆境胁迫[1-5]、花色素苷合成[6-7]、调控开花时间[8]等过程中发挥重要作用。……