鲍锋 赵室梅 杨文 孙刚 李开斌

【摘 要】 目的：建立番石榴叶药材质量标准。方法：通过性状、显微特征和TLC法鉴别不同批次的番石榴叶商品药材;采用HPLC法测定番石榴叶药材指标成分含量;对药材水分、总灰分、酸不溶性灰分及醇溶性浸出物进行测定。结果：番石榴叶药材药显微特征性强;薄层色谱斑点清晰，分离度好，具有较好的专属性;10批番石榴叶样品中，金丝桃苷、异槲皮苷分别在1.017～4.093 mg/g，0.915～2.680 mg/g范围内;药材水分均值为 11.3%，总灰分均值为 5.5%，酸不溶性灰分均值为0.3%，醇溶性浸出物均值为29.2%。结论：该方法准确、简便、灵敏度高、无干扰而且重现性好，适用于番石榴叶药材的质量评价。

【关键词】 番石榴叶;金丝桃苷;异槲皮苷;质量标准

【中图分类号】R284.2   【文献标志码】 A    【文章编号】1007-8517（2021）22-0059-06

Study on Quality Standard of Guava Leaves

BAO  Feng ZHAO Shimei YANG Wen SUN Gang LI Kaibin*

Anshun Institute for food and drug control， Anshun 561000， Ghina

Abstract：Objective To establish the quality standard for guava leaves. Methods the commercial medicinal materials of guava leaves in different batches were identified by character， microscopic characteristics and TLC; the content of index components in guava leaves was determined by HPLC; the moisture， total ash， acid insoluble ash and alcohol soluble extract were determined. Results guava leaves had strong microscopic characteristics， clear TLC spots， good resolution and good specificity. In 10 batches of guava leaves， Hyperoside and Isoquercitrin were in the range of 1.017～4.093 mg/g and 0.915～2.680 mg/g， respectively. The average moisture content of guava leaves was 11.3%， the average total ash content was 5.5%， and the average acid insoluble ash content was 5.5% 3%， and the average value of alcohol soluble extract was 29.2%. Conclusion the method is accurate， simple， sensitive， non-interference and reproducible， which is suitable for the quality evaluation of guava leaves.

Keywords：Guava Leaf; Hyperoside; Lsoquercitrin; Quality Standard

番石榴葉别名鸡矢茶、番桃叶等，为桃金娘科植物番石榴（Psidium guajava Linn）的干燥叶，在沿海及西南诸省均有分布[1-3]。其性平，味涩，微苦。归大肠、肝经，具有燥湿健脾，清热解毒，收敛止泻的功效;主治泻痢腹痛、食积腹胀、齿龈肿痛、风湿痹痛、湿疹臁疮、疔疮肿毒、跌打损伤、外伤出血、蛇虫咬伤等[1-3]。现代药理研究[5-8]表明番石榴叶主要具有降血糖血脂、抗氧化、抗病毒、抗菌抗、肿瘤等活性。番石榴叶主要含萜类、黄酮类、酚类、多糖、挥发油等成分，黄酮类是其重要的活性成分[9-11]，选择黄酮类作为该药材的质量控制指标，以金丝桃苷[12]和异槲皮苷作为质量评价指标，采用HPLC测定其含量，进行质量标准研究，为规范药材质量，保证临床疗效提供科学依据。

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