王庆学 吴雍真 李 倩 杜海洋 刘春艳 柴艺汇

【摘 要】 目的：探讨黄芪治疗2型糖尿病（T2DM）的有效成分及潜在靶点。方法：利用中药系统药理学分析平台数据库（TCMSP）筛选黄芪的有效成分，并收集对应的作用靶点;使用GeneCards、OMIM数据库获取T2DM相关靶点，并筛选黄芪有效成分与T2DM的交集靶点，采用Cytoscape v3.7.2软件和String数据库进行“药物-活性成分-靶蛋白-疾病”及蛋白互作（PPI）网络的构建与分析;通过DAVID数据库对核心靶点进行GO注释和KEGG通路富集分析。结果：筛选出黄芪有效成分20个，与T2DM交集靶点57个。“药物-活性成分-靶蛋白-疾病”网络中Degree排前5的成分为槲皮素、山萘酚、异鼠李素、刺芒柄花素、毛蕊异黄酮。GO功能注释与KEGG通路富集分析共得到116个GO注释，46条KEGG通路，按P值大小，筛选出靠前20个GO注释和KEGG富集通路。结论：结果显示黄芪治疗T2DM的主要有效成分与潜在作用机制具有多成分、多靶点、多通路等特点，为后续深入探讨其具体机制提供思路和基础。

【关键词】 网络药理;黄芪;2型糖尿病;有效成分;靶点1

【中图分类号】R285 【文献标志码】 A 【文章编号】1007-8517（2020）15-0040-07

Abstract：Objective To explore the effective components and potential targets of Astragalus in the treatment of Type 2 diabetes mellitus（T2DM）.Method Screening of active ingredients in Astragalus membranaceus with TCMSP database and collection of corresponding targets. Use GeneCards and OMIM database to obtain T2DM related targets， and select intersection targets of active ingredients of Astragalus and T2DM，Cytoscape v3.7.2 software and String database were used to construct and analyze “drug-active ingredient-target protein-disease” and protein-protein interaction （PPI） network;Gene ontology（GO） annotation and KEGG pathway enrichment analysis of core targets were performed through the DAVID database. Results 20 effective components of Astragalus were screened， and 57 targets were intersected by T2DM.The top 5 components of Degree in the “Drug-Active Ingredients-Target Proteins-Disease” network are quercetin， kaempferol， isorhamnetin， formononetin，Calycosin.GO and KEGG pathway enrichment analysis obtained a total of 116 GO enrichment results， 46 KEGG signaling pathways.Conclusions The results show that the main active ingredients and potential mechanisms of astragalus treatment of T2DM have the characteristics of Multi-component，multiple targets， multiple pathways， etc.， which provides ideas and foundation for further discussion of its specific mechanism.

Keywords：Network Pharmacology;Astragalus Membranaceus;Type 2 Diabetes Mellitus;Active Ingredients;Target

2型糖尿病（Type 2 Diabetes Mellitus;T2DM）是由于胰岛素抵抗与胰岛β细胞功能失常导致碳水化合物、蛋白质、脂肪代谢异常，以高血糖为特征的代谢性疾病[1]。据统计，我国糖尿病患者数量位居世界首位，仅在2015年就约有130万人因糖尿病及其并发症死亡，且其中60岁以下占总死亡人数40.8%，呈逐渐低龄化的趋势，并且国际糖尿病联盟预测，到2040年我国将有1.51亿糖尿病患者[2]。现阶段临床上对T2DM的治疗主要为口服传统降糖药、注射胰岛素等为主，虽可一定程度控制病情，但很难从根本上治愈T2DM[3]。……