张博伦　贾如　胡波　裴丹丹　刘洁　韩如浩　逯宜

[摘要]目的：探讨牙周炎状态下不同咀嚼负荷对牙周组织的炎性状态的影响。方法：取18只大鼠利用局部注射脂多糖（Lipopolysaccharide，LPS）+高糖饮食喂养方法建立实验性动物牙周炎模型，36d后停止处置并随机分为两组：LPS组与LPS+soft组各9只，分别用正常饲料与定制软饲料连续喂养7d，另取3只大鼠作为control组。43d后，采集双侧上颌第一磨牙腭侧龈沟液与牙龈组织及磨牙区上颌骨，分别用于ELISA检测、提取总RNA、反转录后进行Real-time PCR和用于Micro-CT扫描、三维重建后脱钙进行H&E染色。结果：相比于control组，LPS+soft组和LPS组腭侧牙槽骨均出现明显吸收，炎细胞浸润，破骨细胞增殖，形成骨吸收陷窝形成，其中LPS组更为明显。LPS组IL-1β、TNF-α在牙龈组织的表达与龈沟液含量中分泌水平均高于control组，差异具有统计学意义（P<0.05）;牙龈组织中IL-6在LPS组的表达也显著高于control组，差异具有统计学意义（P<0.05）;另外TNF-α在LPS組中的基因表达水平较LPS+soft组显著升高，差异具有统计学意义（P<0.05）。结论：不同硬度的食物会导致牙周组织的炎症反应程度出现差异，较软食物相对正常食物有减轻其炎性状态的作用。

[关键词]牙周炎;牙列缺损;力学载荷;脂多糖;炎症因子

[中图分类号]R781.4+2    [文献标志码]A    [文章编号]1008-6455（2020）08-0023-04

Effects of Inflammation for Periodontitis Tissue on Different Masticatory Loading

ZHANG Bo-lun，JIA Ru，HU Bo，PEI Dan-dan，LIU Jie，HAN Ru-hao，LU Yi

（Department of Prosthodontics，College of Stomatology，Xian Jiaotong University，Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research，Xian 710004，Shaanxi，China）

Abstract： Objective  To explore the effect of different mechanical loads on the inflammatory state of periodontitis tissue. Methods  18 rats were taken to establish an experimental periodontitis model by local injection of LPS （Lipopolysaccharides） + high-sugar feeding method. After 36 days， the injection was stopped and the rats was divided randomly into two groups： the LPS group and the LPS+soft group， there were 9 rats in each group. The two groups were fed respectively with sufficient normal feed and customized soft feed for 7 days. Another 3 rats were taken as control group. After 43 days， the gingival crevicular fluid and the gingival tissue of the palatal side of the maxillary first molars were collected for ELISA detection， total RNA extraction， Real-time PCR after reverse transcription， the maxillary bone of the molar area were collected for H&E staining after Micro-CT scanning and 3D reconstruction. Results  Compared with the control group， the alveolar bone of the LPS+soft group and the LPS group showed obvious resorption， inflammatory cell infiltration， osteoclast proliferation， and Howships lacuna， in particular， the LPS group was more obvious. The expression of IL-1β and TNF-α in gingival tissue and secretion level of gingival crevicular fluid were higher in the LPS group than in the control group， the differences were statistically significant （P<0.05）. The expression of IL-6 in gingival tissue in the LPS group was higher than that in the control group （P<0.05）. In addition， the gene expression level of TNF-α in the LPS group was higher than that in the LPS+soft group （P<0.05）. Conclusion  Different hardness foods will lead to differences in the degree of inflammation of periodontal tissues， the softer foods have a role in reducing their inflammatory status compared to normal diets.

Key words： periodontitis; dentition defect; masticatory loading; lipopolysaccharide; inflammatory factors

牙周炎是口颌系统的常见疾病，为中老年人群牙齿缺失的首要原因[1]。在对患者进行固定或活动义齿修复时，通常需选择余留牙作为基牙，咀嚼力经由基牙牙齿硬组织传导到牙周膜和牙槽骨，进而引发牙周组织相应的生物化学反应。研究显示，健康的牙槽骨在受到力学刺激后会发生成/破骨的双向改变[2]，但对于炎性牙周组织在不同咀嚼负荷下的炎性改变在体研究尚未见报道。学者们常通过咬合早接触诱导咬合创伤建立动物模型，而该方法会引起大鼠咬合痛[3-4]，并非生理状态下的过大牙合力状态。……