范婧莹 娄余 张娜 彭瑶 朱莹

〔摘要〕 目的 探討三物白散对Survivin-RNA基因沉默转染胃癌SGC-7901细胞凋亡、迁移的影响。方法 将实验分成6组：空白对照组、LV-RNAi 组、Survivin-RNAi-LV组、空白对照+中药组、LV-RNAi+中药组、Survivin-RNAi-LV+中药组;采用流式细胞仪检测各组细胞的凋亡，划痕实验检测各组细胞的迁移能力。结果 细胞凋亡率：Survivin-RNAi-LV组明显高于空白对照组和LV-RNAi组（P<0.05）;Survivin-RNAi-LV+中药组与空白对照+中药组、LV-RNAi+中药组比较明显较高（P<0.05）;Survivin-RNAi-LV+中药组高于Survivin-RNAi-LV组，差异具有统计学意义（P<0.05）。胃癌细胞的24 h迁移率：Survivin-RNAi-LV组明显低于空白对照组和LV-RNAi组（P<0.05）;Survivin-RNAi-LV+中药组与空白对照+中药组、LV-RNAi+中药组比较，其结果明显低于后两组（P<0.05）;Survivin-RNAi -LV+中药组与Survivin-RNAi-LV组比较，细胞迁移率明显降低，差异有统计学意义（P<0.05）。结论 Survivin基因沉默转染，增强了三物白散诱导胃癌SGC-7901细胞凋亡和抑制其迁移的作用。

〔关键词〕 Survivin;三物白散;胃癌;细胞凋亡;迁移

〔中图分类号〕R285.5;R735.2       〔文献标志码〕A       〔文章编号〕doi：10.3969/j.issn.1674-070X.2019.08.002

〔Abstract〕 Objective To investigate the effects of Sanwubai Powder on apoptosis and migration of gastric cancer SGC-7901 cells transfected with Survivin-RNA gene silencing. Methods The experiment was divided into 6 groups： a blank control group， a LV-RNAi group， a Survivin-RNAi-LV group， a blank control group + TCM group， a LV-RNAi + TCM group， a Survivin-RNAi -LV + TCM group. The cell apoptosis was observed by flow cytometry， and the cell migration was assessed by cell scratch experiment. Results The apoptotic rate of the Survivin-RNAi -LV group was significantly higher than that of blank control group and LV-RNAi group （P<0.05）; The apoptosis rate of the Survivin-RNAi-LV + TCM group was significantly higher than that of the blank control group + TCM group and the LV-RNAi + TCM group （P<0.05）; The Survivin-RNAi-LV+ TCM group was higher than the Survivin-RNAi-LV group， and the difference was statistically significant （P<0.05）. The 24 hour cell migration rate in the Survivin-RNAi -LV group was significantly lower than that in the blank control group and the LV-RNAi group （P<0.05）. The Survivin-RNAi -LV + TCM group was significantly lower than that of the blank control group + TCM group and the LV-RNAi + TCM group （P<0.05）; Compared with the Survivin-RNAi-LV group， the cell migration rate of the Survivin-RNAi-LV + TCM group was significantly decreased， and the difference was statistically significant （P<0.05）. Conclusion After Survivin gene silencing transfection， the sensitivity of SGC-7901 cells to Sanwubai Powder was significantly enhanced， which further promoted the apoptosis of SGC-7901 cells and inhibited their migration.

〔Keywords〕 Survivin; Sanwubai Powder; gastric cancer; apoptosis; migration

胃癌是常见的消化道恶性肿瘤之一，其高发病率及死亡率严重威胁了人类的健康。我国是胃癌的高发区，胃癌的发病例数和死亡例数分别占全球胃癌发病和死亡的42.6%和45.0%[1]。中医药对肿瘤有着明确的治疗效果，且毒副作用小，但与常规化疗药物相比，中药抗肿瘤的效果一般[2]，寻找新的途径提高中药的抗肿瘤效果，具有重要的研究意义。我们前期研究发现[3-4]，三物白散可抑制胃癌细胞的增殖，诱导其凋亡，其机制可能与抑制Survivin的表达有关。RNA干扰（RNA interference， RNAi）是抑制靶基因表达的核酸操作技术，广泛应用于肿瘤基因治疗的研究[5-6]。有研究发现，胃癌MGC-803细胞经Cdx2基因沉默转染后，对人参黄芪复方的敏感性明显增强，进而促进了胃癌细胞的凋亡等[2]。……