饶晨晨 高文雅 陶仕英 崔丽霞 石丹宁 牛建昭 赵丕文 孙丽萍 王燕霞

〔摘要〕 目的 观察二仙汤对卵巢早衰（premature ovarian failure， POF）大鼠的抗氧化應激效应。方法 雌性SD大鼠40只，监测一周动情周期，筛选有正常动情周期的大鼠入组，随机分为4组：正常组、模型组、阳性药组和二仙汤组。除正常组外，其余3组使用顺铂注射液造POF模型，阳性药组和二仙汤组在造模同时进行药物干预，灌胃期间监测动情周期，15 d后麻醉取材，应用放免法检测血清性激素卵泡刺激素（follicle timulating hormone， FSH）、黄体生成素（luteinizing hormone， LH）及雌激素（estrogen， E2）的水平以及氧化应激相关指标丙二醛（malondialdehyde， MDA）、抗氧化指标超氧化物歧化酶（superoxide dismutase， SOD）和过氧化氢酶（catalase， CAT）的含量，显微镜下观察各组卵巢组织的病理改变。结果 与正常组相比较，模型组大鼠动情周期及动情间期明显延长（P<0.01），血清FSH、LH、MDA含量显著升高（P<0.01），E2、SOD及CAT水平显著降低（P<0.01），卵巢组织中闭锁卵泡增多，窦卵泡数减少。与模型组相比，阳性药组与二仙汤组动情周期缩短（P<0.05或P<0.01），血清FSH、LH、MDA含量显著降低（P<0.05或P<0.01），E2、SOD及CAT的含量显著上升（P<0.05或P<0.01），闭锁卵泡数量减少，窦卵泡数增多。结论 二仙汤能通过调节POF大鼠的的抗氧化能力来改善卵巢功能，减少氧化应激带来的损伤从而治疗POF。

〔关键词〕 卵巢早衰;二仙汤;氧化应激;雌激素;黄体生成素

〔中图分类号〕R285.5;R711.75       〔文献标志码〕A       〔文章编号〕doi：10.3969/j.issn.1674-070X.2019.05.004

〔Abstract〕 Objective To investigate the anti-oxidative stress effect of Erxian Decoction on rats with premature ovarian failure（POF）. Methods A total of 40 female SD rats were monitored the estrus cycle and then chose the rats that had the normal estrus cycle into the experiment. The rats were randomly divided into 4 groups： a normal group， a model group， a positive drug group and an Erxian Decoction group. Except the normal group， the other 3 groups were made POF model by using cisplatin injection. The positive drug group and the Erxian Decoction group were given drug intervention at the same time， and estrus cycle was monitored during gastric administration. After 15 days， anesthesia and killed， the levels of follicle stimulating hormone （FSH）， luteinizing hormone （LH）， estrogen （E2）， malondialdehyde （MDA）， superoxide dismutase （SOD） and catalase （CAT） were measured by radioimmunoassay， and the pathological changes of ovarian tissues in each group were observed under microscope. Results Compared with the normal group， the estrous cycle and estrous interval in the model group were significantly prolonged （P<0.01）. The levels of FSH， LH and MDA were significantly increased （P<0.01）， and the levels of E2， SOD and CAT were significantly decreased （P<0.01）. Atresia follicles in ovarian tissue increased， and the number of antral follicles decreased. Compared with the model group， the estrus cycle in the positive drug group and the Erxian Decoction group was shortened （P<0.05 or P<0.01）， and the serum FSH， LH and MDA levels were significantly decreased （P<0.05 or P<0.01）. The levels of E2， SOD and CAT were significantly increased （P<0.05 or P<0.01）， and the number of atresia follicles and sinus follicles were decreased in the positive drug group and Erxian Decoction group， and the number of sinus follicles was increased in the positive drug group and the Erxian Decoction group. Conclusion Erxian Decoction can improve ovarian function by regulating the antioxidant capacity of POF rats and reduce the damage caused by oxidative stress， and then cure POF.

〔Keywords〕 premature ovarian failure; Erxian Decoction; oxidative stress; estrogen; luteinizing hormone

卵巢早衰（premature ovarian failure， POF）是指育齡期女性卵巢功能提前衰退，出现闭经和性器官萎缩，以卵泡刺激素（follicle timulating hormone， FSH）和黄体生成素（luteinizing hormone， LH）水平升高、雌激素（estrogen， E2）降低为特征的一种妇科内分泌性疾病[1]。POF患者主要表现为月经失调、闭经、不孕、性功能降低、围绝经期综合征以及烘热汗出、腰膝冷痛、生殖器及第二性征逐渐退化的类更年期症状等一系列症状。POF的发病率为1%～3%[2]，随着生活压力的逐渐增加，POF近些年来的发病率呈上升趋势。目前对于POF的治疗，西医多采用雌孕激素替代疗法[3]，要起到治疗作用需要长期服药，且停药后容易复发。

顺铂是临床上常用的化疗药物，顺铂有较强的细胞毒性，在杀死肿瘤细胞的同时也对正常细胞造成损伤，顺铂对细胞的损伤主要是通过造成DNA损伤实现的。……