刘晓华

[摘要] 目的 探讨在梅毒螺旋体（TP）特异性抗体检验中罗氏电化学发光免疫法的应用价值。 方法 方便选取医院2016年2月—2017年9月收治的240例高危TP特异性抗体阳性患者的临床资料进行回顾性分析，均采用甲苯胺红不加热血清试验（TRUST）、快速血浆反应素环状卡片试验（RPR）和梅毒螺旋体血凝试验（TPHA）进行检验，并利用罗氏电化学发光免疫法检测TP特异性抗体。评价罗氏电化学发光免疫法的临床应用价值。 结果 240例患者中经证实共有51例TP特异性抗体阳性，阳性率为21.25%；在51例证实TP特异性抗体阳性患者中罗氏电化学发光免疫法检测50例阳性；在189例经证实TP特异性抗体阴性受检者中罗氏电化学发光免疫法检测187例阴性，符合率为98.75%，灵敏度、特异度、阳性和阴性预测值分别为98.04%、98.94%、96.15%和99.47%。 结论 对TP特异性抗体检验中罗氏电化学发光免疫法准确度、灵敏度、特异度、阳性和阴性预测值均理想，临床应用的价值较高。

[关键词] 罗氏电化学发光免疫法；梅毒螺旋体；特异性抗体

[中图分类号] R446.6；R759.1 [文献标识码] A [文章编号] 1674-0742（2018）08（a）-0182-03

Application of Roche Electrochemiluminescence Immunoassay in the Examination of Treponema pallidum-Specific Antibodies

LIU Xiao-hua

Emergency Department， Third People's Hospital of Jinan， Jinan， Shandong Province， 250132 China

[Abstract] Objective To evaluate the value of Roche electrochemiluminescence immunoassay in the detection of specific antibodies against Treponema pallidum （TP）. Methods The clinical data of 240 cases of high-risk TP-specific antibody positive patients admitted from February 2016 to September 2017 in the hospital were convenient selected and retrospectively analyzed using the toluidine red unheated serum test （TRUST） and rapid plasma renin card test （RPR） and the Treponema pallidum hemagglutination test （TPHA） were performed and Roche-specific electrochemiluminescence immunoassay was used to detect TP-specific antibodies. To evaluate the clinical application value of Roche electrochemiluminescence immunoassay. Results A total of 51 cases of TP-specific antibodies were confirmed in 240 patients， with a positive rate of 21.25%； 50 cases were positive for TP-specific antibodies in 51 cases； Roche electrochemiluminescence immunoassay tested 50 cases positive； 189 cases were confirmed TP Among 187 patients with specific antibody negative test， 187 cases were negative by Roche electrochemiluminescence immunoassay， the coincidence rate was 98.75%， and the sensitivity， specificity， positive， and negative predictive values were 98.04%， 98.94%， 96.15%， and 99.47%， respectively. Conclusion The accuracy， sensitivity， specificity， positive and negative predictive value of Roche electrochemiluminescence immunoassay in the detection of TP-specific antibodies are all satisfactory， and the value of clinical application is high.

[Key words] Roche electrochemiluminescence immunoassay； Treponema pallidum； Specific antibodies

梅毒螺旋體（TP）是性传播疾病梅毒的病原体，又被称为苍白螺旋体。梅毒属于广泛流行的性病之一，在我国的发病率有所回升，且对人类健康的危害十分严重。在梅毒发生的初期，TP侵入皮肤黏膜大约3周后，局部可出现无痛性硬结及溃疡，溃疡渗出物的传染性极强，其中含有大量的TP；在梅毒中期，患者以全身皮肤黏膜梅毒疹、淋巴结肿大，亦或累及骨、关节、眼部甚至是其它器官等为主要临床症状；……