袁轶峰 傅显文 朱文雄

摘要：目的 觀察益气活血消癥方对良性前列腺增生大鼠细胞凋亡的影响，初步探讨其作用机制。方法 将60只8周龄雄性SD大鼠随机分为正常组、阳性对照组和消癥方低、中、高剂量组，每组10只。除正常组外，其余各组大鼠均予去势加丙酸睾酮注射法复制前列腺增生模型。正常组和模型组大鼠予生理盐水灌胃，消癥方低、中、高剂量组大鼠分别予益气活血消癥方10、20、40 mg/100 g灌胃，阳性对照组大鼠予癃开颗粒混悬液11 mg/100 g灌胃，1次/d，连续30 d。末次给药24 h后，脱颈处死大鼠，取前列腺组织，免疫组化检测大鼠前列腺组织Bcl-2、Bax蛋白表达。结果 与正常组比较，模型组大鼠前列腺组织Bax表达显著降低，Bcl-2表达显著升高；与模型组比较，各给药组大鼠前列腺组织Bax表达显著升高，Bcl-2表达显著降低，且消癥方高剂量组效果最优。结论 益气活血消癥方可促进大鼠前列腺组织细胞凋亡，其机制与前列腺组织Bax蛋白表达升高、Bcl-2蛋白表达降低相关，从而改善良性前列腺增生。

关键词：益气活血消癥方；良性前列腺增生；细胞凋亡；Bax；Bcl-2；大鼠

DOI：10.3969/j.issn.1005-5304.2018.09.013

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2018）09-0052-04

Abstract： Objective To investigate the effects of Yiqi Huoxue Xiaozheng Formula on cell apoptosis in rats with benign prostatic hyperplasia （BPH）； To preliminarily explore its mechanism of action. Methods Totally sixty 8-week-old male SD rats were randomly devided into normal group， positive control group， Yiqi Huoxue Xiaozheng Formula low-， medium-， and high-dose groups， with 10 rats in each group. Except for the rats in normal group， rats in other groups were treated with castrate and testosterone propionate injection to replicate BPH model. The normal group and the model group were given normal saline for gavage； Yiqi Huoxue Xiaozheng Formula low-， middle-， and high-dose groups were given Yiqi Huoxue Xiaozheng Formula 10， 20， 40 mg/100 g for gavage， respectively； rats in positive control group were given Longkai Granule suspension 11 mg/100 g for gavage， once a day， for 30 d. Twenty-four hours after the last administration， the rats were sacrificed by cervical dislocation. The prostate tissue was taken. The protein expressions of Bcl-2 and Bax in the prostate tissue were detected by immunohistochemistry. Results Compared with the normol group， the expression of Bax in the prostate tissue of the model group decreased significantly， while the expression of Bcl-2 increased significantly； Compared with the model group， the expression of Bax in the prostate tissue of rats in administration groups increased significantly， while the expression of Bcl-2 was significantly reduced， and Yiqi Huoxue Xiaozheng Formula high-dose group was superior to other groups. Conclusion Yiqi Huoxue Xiaozheng Formula can improve benign prostatic hyperplasia by promoting the apoptosis of rat prostate tissue， and its mechanism is related to the increase of the expression of Bax protein and the decrease of Bcl-2 protein expression in prostate tissue.

Keywords： Yiqi Huoxue Xiaozheng Formula； benign prostatic hyperplasia； apoptosis； Bax； Bcl-2； rats

良性前列腺增生（benign prostatic hyperplasia，BPH）是男科的常见病，以尿频、夜尿次数增多、排尿困难为主要临床表现，控制不佳者，可并发尿潴留或尿失禁，甚至肾功能受损等疾患[1]。目前对于BPH的发病机制尚未定论，其治疗方式以观察等待、药物保守治疗及外科手术为主[2]。临床常用治疗药物包括5α-还原酶抑制剂、激素、α受体阻滞剂等，因这些药物的不良反应及耐药性，使其临床应用受到限制；……