萨依腊西·杰恩斯努尔 肖岚 龙见汝

摘要：目的 观察补肾活血针刺法对阿尔茨海默病（AD）模型SAMP8小鼠行为学及海马内源性神经干细胞增殖的影响。方法 将18只7月龄雄性SAMP8小鼠作为AD模型，随机分为针刺组、非穴组和模型组，另取同月龄雄性SAMR1小鼠6只作为正常组。针刺组小鼠选取“肾俞”“百会”“血海”“膈俞”进行针刺干预，非穴组小鼠取双侧肋下固定非穴点进行针刺，模型组和正常组小鼠予相同程度捉抓刺激，连续干预8周。采用水迷宫实验检测小鼠学习记忆能力，免疫组化检测海马BrdU阳性细胞数。结果 与正常组比较，模型组小鼠逃避潜伏期明显延长（P<0.05），停留在原平台象限时间明显缩短（P<0.05），BrdU阳性细胞数明显减少（P<0.05）；与模型组、非穴组比较，针刺组小鼠逃避潜伏期明显缩短（P<0.05），停留在原平台象限时间明显延长（P<0.05），BrdU阳性细胞数明显增加（P<0.05）。结论 补肾活血针刺法可能通过诱导海马内源性神经干细胞的增殖，改善AD模型SAMP8小鼠的学习记忆能力。

关键词：补肾活血；针刺；阿尔茨海默病；神经干细胞增殖；SAMP8小鼠

DOI：10.3969/j.issn.1005-5304.2018.09.009

中图分类号：R245 文献标识码：A 文章编号：1005-5304（2018）09-0035-04

Abstract： Objective To investigate the effects of Bushen Huoxue acupuncture method on the behavioral changes and the proliferation of endogenous neural stem cells （NSCs） in hippocampus of Alzheimer disease （AD） model mice. Methods 18 male SAMP8 mice， seven months old， were randomly divided into acupuncture group， non-acupoint control group， and model control group. And another age-matched 6 male SAMR1 mice were prepared as normal control group. Mice in acupuncture group were intervened by acupuncture method in the acupoints of “Shenshu”， “Baihui”， “Xuehai”， and “Geshu”. Mice in non-acupoint control group were treated by stimulating the fixed non-point under the bilateral rib， while mice in model control group and normal control group were raised without special treatment but administered the stimulation of catching with the same time and the same stimulus intensity. All treatrment lasted for 8 weeks. After the intervention， the learning and memory abilitities and brain hippocampus BrdU positive cells of all groups were detected. Results Compared with the nomal control group， mice in the model control group had longer escape latency and less time spent in former platform quadrant （P<0.05）； the number of BrdU positive cells decreased significantly （P<0.05）. Compared with the model control group and the non-acupoint control group， mice in the acupuncture group had shorter escape latency and more time spent in former platform quadrant （P<0.05）； the number of BrdU positive cells increased significantly （P<0.05）. Conclusion Bushen Huoxue acupuncture method can improve the learning and memory abilities of SAMP8 mice AD model by inducing the proliferation of hippocampal endogenous NSCs.

Keywords： Bushen Huoxue； acupuncture method； Alzheimer disease； NSCs proliferation； SAMP8 mice

阿尔茨海默病（Alzheimer disease，AD）是一种与年龄相关的复杂性神经性疾病，以认知功能进行性减退为特征[1]。本课题组前期研究发现，补肾活血法对AD认知障碍和病情发展有一定的控制作用[2]；补肾活血针刺法通过抗氧化应激、调节β淀粉样蛋白代谢、抑制神经反应及修复细胞骨架等方面的作用改善AD模型小鼠的学习记忆能力[3-4]。神经干细胞（neural stem cell，NSCs）是一类具有自我产生新细胞及分化能力的多能干细胞，其相关研究已成為神经学科领域热点。本实验在前期研究基础上，采用Morris水迷宫实验和免疫组化方法，观察补肾活血针刺法对AD模型SAMP8小鼠行为学及海马内源性NSCs增殖的影响。

1 材料与方法

1.1 动物及分组

清洁级雄性7月龄SAMP8小鼠18只、SAMR1小鼠6只，体质量（28±1.5）g，购于天津中医药大学第一附属医院动物实验中心，动物质量合格证号0001353。……