巢蕾　曹雨诞　陈佩东

[摘要] 目的 研究厚樸以及厚朴酚促进小鼠胃肠动力的作用机制。 方法 将304只雄性ICR小鼠按体重随机分为厚朴提取物低、中、高剂量组（1、2、4 g/kg），厚朴酚低、中、高剂量组（40、80、160 mg/kg），阳性对照组（盐酸依托比利50 mg/kg）及空白对照组，每组8只。分别测定正常小鼠小肠推进率、采用阿托品（1 mg/kg）及昂丹司琼（1.6 mg/kg）造模后小鼠胃排空和小肠推进率，应用多通道生理仪测定小鼠电生理波形。 结果 与空白对照组比较，厚朴提取物中、高剂量组与厚朴酚中、高剂量组均可显著提高正常小鼠小肠推进率（P < 0.05）。与阿托品模型组比较，厚朴提取物中、高剂量组和厚朴酚低、中、高剂量组小鼠胃排空率均显著上升，差异有统计学意义（P < 0.05或P < 0.01）。与阿托品模型组比较，厚朴提取物高剂量组和厚朴酚高剂量组小肠推进率均显著上升，差异有统计学意义（P < 0.05）。与昂丹司琼模型组比较，厚朴酚高剂量组胃排空率、小肠推进率显著升高，差异有统计学意义（P < 0.05）。 结论 厚朴提取物及厚朴酚可提高小鼠胃排空率及小肠推进率，其对胃肠动力的促进作用可能与5羟色胺（5-HT3）受体和M胆碱受体相关。

[关键词] 厚朴；胃动力；小肠推动力；厚朴酚

[中图分类号] R57 [文献标识码] A [文章编号] 1673-7210（2018）05（a）-0031-04

Study on the effect of Magnoliae Officinalis Cortex on gastrointestinal motility disorders

CHAO Lei1 CAO Yudan2 CHEN Peidong2 ZHANG Li2

1.Department of Pharmacy， Jiangsu Hospital of Traditional Chinese Medicine， Jiangsu Province， Nanjing 210029， China； 2.College of Pharmacy， Nanjing University of Chinese Medicine， Jiangsu Province， Nanjing 210046， China

[Abstract] Objective To investigate the mechanism of Magnoliae Officinalis Cortex on promoting gastrointestinal motility of mice. Methods Three hundred and four male ICR mice were randomly divided into low， medium， high dose of Magnoliae Officinalis Cortex extract groups （1， 2， 4 g/kg）， low， medium， high dose of magnolol groups （40， 80， 160 mg/kg）， positive control group （Etobil Hydrochloride， 50 mg/kg） and blank control group according to the body weight， with 8 mice in each group. The intestinal propulsive rate of normal mice， and the gastric empty and intestinal propulsive rate of Atropine （1 mg/kg） and Ondansetron （1.6 mg/kg） induced mice were measured respectively. The multi-channel physiological instrument was used to detect the muscle electrophysiological waveform. Results Compared with blank control group， the intestinal propulsive rates of normal mice in medium， high dose of Magnoliae Officinalis Cortex extract groups and medium， high dose of magnolol groups were significantly improved （P < 0.05）. Compared with Atropine model group， the gastric emptying rates in medium， high dose of Magnoliae Officinalis Cortex extract groups and low， medium， high dose of magnolol groups were significantly improved （P < 0.05 or P < 0.01）. Compared with Atropine model group， the intestinal propulsive rates in high dose of Magnoliae Officinalis Cortex extract group and high dose of magnolol group were improved， the differences were statistically significant （P < 0.05）. Compared with Ondansetron model group， the gastric emptying rate and intestinal propulsive rate in high dose of magnolol group were improved， the differences were statistically significant （P < 0.05）. Conclusion Magnoliae Officinalis Cortex extract and magnolol can improve the gastric emptying rate and intestinal propulsive rate of mice， the positive effect for gastrointestinal motility may be connected with 5-HT3 receptor and M acetylcholine receptor.

[Key words] Magnoliae Officinalis Cortex； Gastric motility； Small intestinal propulsion； Magnolol

厚朴为木兰科植物厚朴Magnolia officinalis Rehd.et Wils.或凹叶厚朴Magnolia officinalis Rehd.et Wils.var.biloba Rehd.et Wils.的干燥干皮、根皮及枝皮[1]，主要化學成分为新木脂素类[2-3]、苷类[4]和生物碱类[5]。厚朴主要的功效为消胀除满、化导消积[6]，为通降胃气之要药[7]。此外，厚朴还具有抗氧化[8]、抗抑郁等作用[9]。厚朴对胃肠动力障碍患者具有确切的疗效。厚朴排气合剂对术后患者具有明显的改善胃肠动力的作用[10]，厚朴提取物可提高胃排空率并且促进小肠推进率，对血清中D-木糖、胃动素、胃泌素等水平有调节作用[11]，而厚朴中的主要成分厚朴酚对胃排空率及小肠推进率均有促进作用[12]。尽管厚朴及含有厚朴的复方对胃肠动力障碍的作用研究有一定进展，但是其作用机制并不明确。在前期的工作中我们对厚朴的炮制工艺及化学成分进行了研究[13]，本研究应用M胆碱受体拮抗药阿托品和5羟色胺（5-HT3）受体拮抗剂昂丹司琼复制了小鼠胃肠动力障碍模型，对厚朴提取物和其中主要成分厚朴酚促进小鼠胃肠动力的作用进行了研究。……