孙红炜 徐晓辉 李凡 高瑞 杨淑珂 路兴波

摘要：本試验用田间接虫和室内生测方法，研究国产转基因玉米杂交种瑞丰1号-双抗12-5（RF1-12-5）对3种主要的玉米鳞翅目害虫亚洲玉米螟、黏虫、棉铃虫的抗性。田间结果表明：RF1-12-5对亚洲玉米螟、棉铃虫的抗性在相应的鉴定时期均达到高抗水平，RF1-12-5心叶期对黏虫的抗性为抗性水平。室内鉴定结果表明：心叶期，RF1-12-5对亚洲玉米螟抗性起效较慢，饲喂48～72 h后校正死亡率分别为63.97%、77.14%、88.00%；吐丝期饲喂48 h后校正死亡率达100%，抗性显著；籽粒期饲喂24、48、72 h后校正死亡率分别为68.86%、83.96%、98.03%。心叶期饲喂24 h后对黏虫基本无抗性效果，但48、72 h后的校正死亡率分别达到94.64%、100%。吐丝期饲喂24 h后对棉铃虫抗性达到87.59%，48 h后对棉铃虫校正死亡率达到100%，抗性较强。总体来看，RF1-12-5田间对亚洲玉米螟、棉铃虫抗性较高，对黏虫抗性略低；室内心叶期对亚洲玉米螟抗性较弱，但在其他时期对亚洲玉米螟、黏虫、棉铃虫均表现了较好的抗性。因此RF-12-5具有推广应用的潜力。

关键词：抗虫耐除草剂玉米；瑞丰1号-双抗12-5；鳞翅目害虫；田间抗虫性；室内抗虫性

中图分类号：S513.037文献标识号：A文章编号：1001-4942（2018）05-0109-06

Abstract Through filed experiments and indoor bioassay， the resistances of domestic transgenic maize hybride Ruifeng 1-Double Resistance 12-5（RF1-12-5） against three major maize lepidopteran pests， Pyrausta nubilalis， Mythimna separate and Helicoverpa armigera were studied. The results of the filed experiments showed that the resistance of RF1-12-5 against Ostrinia furnacalis and Helicoverpa armigera were in high level during the corresponding identification periods. The resistance against Mythimna separate was in moderate level during the whorl stage. The indoor bioassay results showed that RF1-12-5 had slower resistance against Ostrinia furnacalis at whorl stage. After feeding with RF1-12-5 leaves for 24，48 and 72 hours， the corrected mortality rates were respectively 63.97%， 77.14% and 88.00%. At filamentous stage， the resistance was significant and the corrected mortality rate reached 100% after 48 hours. During the grain period， the corrected mortality rates were respectively 68.86%， 83.96% and 98.03% after 24，48 and 72 hours. For Mythimna separate， at whorl stage， there was no obvious resistant effect after 24 hours， but the corrected mortality rates were 94.64% and 100% respectively after 48 and 72 hours. For Helicoverpa armigera， RF1-12-5 showed obvious resistance at filamentous stage and the corrected mortality rates were 87.59% and 100% after 24 and 48 hours respectively. In all，RF1-12-5 showed obvious field resistance against Ostrinia furnacalis and Helicoverpa armigera，but lower resistance against Mythimna separate. It showed lower resistance against Ostrinia furnacalis at whorl stage during indoor bioassay， but better resistance against Ostrinia furnacalis， Mythimna separate and Helicoverpa armigera at other stages. We concluded that RF1-12-5 had the potential for popularization and application in the future.

Keywords Insect-and herbicide-resistant maize； Ruifeng 1-Double Resistance 12-5； Lepidopteran pests； Field insect resistance； Indoor insect resistance

玉米是我国重要的粮食作物和加工原料，在我国粮食生产中占据重要位置。亚洲玉米螟、黏虫和棉铃虫是影响玉米产量的三大主要鳞翅目害虫，在个别年份，害虫的为害甚至会造成玉米绝收[1，2]。长期以来，农业生产中均采用化学方法对鳞翅目害虫进行防治。但化学防治对环境危害大，同时存在防治不及时的缺点。随着生物技术……