郭瑞杰 李文 刘风珍 张昆 万勇善

摘要： DREB1 （dehydration responsive element binding protein） 是植物中特有的一类转录因子，在调控与逆境诱导相关基因的表达、参与植物对盐胁迫的响应及适应过程中具有重要作用。本研究以转AhDREB1基因T2代花生植株及其受体品种丰花2号（对照）为材料，苗期用1% NaCl 进行盐胁迫处理，分析胁迫前后植株表型和基因表达变化，并对SOD、POD活性和MDA、可溶性蛋白含量等生理指标进行测定。结果表明：盐胁迫下，转基因植株叶片保绿程度及根系生长状况明显好于对照。转基因植株DREB1基因表达量升高、响应盐胁迫速度比对照快。转基因植株参与盐胁迫响应的SOD、POD活性和可溶性蛋白含量高于对照，MDA含量低于对照，说明AhDREB1基因过量表达能有效提高花生的耐盐性。筛选出耐盐性较好的转基因单株4个，分别是D254、D380、D385、D448。

关键词：花生；DREB1；表型；表达量；生理指标；耐盐性

中图分类号：S565.201文献标识号：A文章编号：1001-4942（2018）06-0072-07

Abstract As a specific transcription factor in plants， DREB1 （dehydration responsive element binding protein） plays an important role in regulating the expression of stress-induced related genes and involves in the response and adaptation of plants to salt stress. In this study， T2 transgenic peanut plants of AhDREB1 and its receptor Fenghua 2 （control） were used as the materials. The seedlings were conducted salt stress treatment with 1% NaCl， and the changes of phenotype and gene expression before and after stress treatment were analyzed. The physiological indexes such as activities of SOD and POD， contents of soluble protein and MDA were measured. The results showed that under salt stress， the green degree and root growth of transgenic plants were better than those of control. The expression level of DREB1 in transgenic plants increased and the response to salt stress was faster than that of control. The activities of SOD and POD and the content of soluble protein of transgenic plants were higher than those of control， and the content of MDA was lower. These results indicated that overexpression of AhDREB1 could enhance the salt tolerance of transgenic peanut plants. Four transgenic plants with better salt tolerance were screened， namely D254， D380， D385 and D448.

Keywords Peanut； DREB1； Phenotype； Expression quantity； Physiological index； Salt tolerance

鹽碱土在全球范围内分布广泛，约有 100×108 hm2土地受到不同程度盐渍化的威胁[1]。我国盐渍土地面积约占全球的1/10[2]，占全国耕地面积的6.2%。土地次生盐碱化面积的不断增加已成为制约我国农业可持续发展的重要因素之一。花生是重要的油料、经济和蛋白质作物，目前关于花生耐盐及盐胁迫下体内离子平衡调节及适应机制的相关研究较少[3]。利用现代生物技术手段培育高耐盐碱花生新品种对盐碱地开发利用、增加农民收入具有重要意义。

DREB是一类植物特有且含有1个高度保守AP2结构域的转录因子，属于 AP2/EREBP（AP2/ethylene-responsive element-binding proteins，乙烯应答元件结合蛋白）家族。……