古丽那尔·阿布拉江　桑伟　王雯

[摘要] 目的 探討E-钙黏蛋白（E-cadherin）和Claudin-7在宫颈鳞状细胞癌组织中的表达及其与临床病理特征的关系。 方法 以2016年8月～2017年8月新疆医科大学第一附属医院采集的正常宫颈上皮组织（对照组，n=30）、重度宫颈上皮内瘤变组织（HSIL组，n=28）和宫颈鳞状细胞癌组织（CA组，n=80）为研究样本，采用免疫组织化学方法检测三组E-cadherin、Claudin-7蛋白表达情况，分析两者与宫颈鳞状细胞癌病理特征的关系，同时对两者的表达水平进行相关性分析。 结果HSIL组、CA组E-cadherin蛋白阳性表达率明显低于对照组，且HSIL组明显高于CA组（P < 0.05）；HSIL组、CA组Claudin-7蛋白阳性表达率明显低于对照组，HSIL组明显低CA组（P < 0.05）；E-cadherin蛋白阳性表达率与宫颈鳞状细胞癌肿瘤大小、淋巴结转移呈负相关（P < 0.05），与组织分化程度呈正相关（P < 0.05），Claudin-7蛋白阳性表达率与淋巴结转移呈正相关（P < 0.05），与组织分化程度、临床分期呈负相关（P < 0.05）；Claudin-7蛋白与E-cadherin蛋白表达呈负相关（P < 0.05）。 结论 宫颈鳞状细胞癌组织中E-cadherin和Claudin-7蛋白阳性表达率分别明显降低和升高，两者阳性表达率与宫颈鳞状细胞癌临床病理特征紧密相关。

[关键词] 宫颈鳞状细胞癌；E-钙黏蛋白；Claudin-7；病理特征；相关性

[中图分类号] R737.33 [文献标识码] A [文章编号] 1673-7210（2018）04（b）-0095-04

[Abstract] Objective To investigate the expression of E-cadherin and Claudin-7 in cervical squamous cell carcinoma and its relationship with clinicopathological features. Methods The normal cervical epithelial tissues （control group， n=30）， high squamous intraepithelial neoplasias （HSIL group， n=28） and cervical squamous cell carcinomas （CA group， n=80） in the First Affiliated Hospital of Xinjiang Medical University from August 2016 to August 2017 were selected as the research samples. The expression of E-cadherin and Claudin-7 protein in the three groups was detected by the immunohistochemical method. The relationship between them and pathological characteristics of cervical squamous cell carcinoma was analyzed， and the correlation between the expression levels of the two was also analyzed. Results The positive expression rate of E-cadherin protein was significantly higher in the control group than in HSIL group and CA group， and significantly higher in HSIL group than in CA group （P < 0.05）. The positive rate of Claudin-7 protein was significantly lower in the control group than in HSIL group and CA group， and significantly lower in HSIL group than in CA group （P < 0.05）. The positive expression rate of E-cadherin protein was negatively correlated with the size and lymph node metastasis of cervical squamous cell carcinoma （P < 0.05）， and positively correlated with the differentiation degree （P < 0.05）. The positive expression rate of Claudin-7 protein was significantly positively correlated with lymph node metastasis （P < 0.05）， and negatively correlated with the differentiation degree and clinical stage （P < 0.05）. The expression of Claudin-7 protein was negatively correlated with the expression of E-cadherin protein （P < 0.05）. Conclusion The positive expression rates of E-cadherin and Claudin-7 protein in cervical squamous cell carcinoma are significantly decreased and increased， respectively. The positive expression rate of them is closely related to the clinicopathological features of cervical squamous cell carcinoma.

[Key words] Cervical squamous cell carcinoma； E-cadherin； Claudin-7； Pathological features； Correlation

宫颈癌为全球范围内女性中常见恶性肿瘤之一，目前对其病因尚未完全明确，多认为与早孕、早产、多育、吸烟等因素有关[1]。随医学技术的不断更新及普及工作的不断开展，虽宫颈癌早期筛查和治疗日益被重视，但宫颈癌患病率仍居高不下[2]。宫颈癌约有80%为宫颈鳞癌[3]，临床有必要积极探究灵敏的肿瘤标志物对宫颈鳞状细胞癌发生、进展进行有效评估。早期有学者研究表明E-钙黏蛋白（E-cadherin）可有效评估肿瘤细胞侵袭及迁移能力[4]，而临床实践表明Claudin-7在癌组织中异常表达[5]，因而笔者推测E-cadherin和Claudin-7表达与宫颈鳞状细胞癌临床病理特征有一定关系，并与于本文展开临床对照性研究。

1 资料与方法

1.1 一般资料

以2016年8月～2017年8月新疆医科大学第一附属医院（以下简称“我院”）病理科采集的正常宫颈上皮组织（对照组，n=30）、重度宫颈上皮内瘤变组织（HSIL组，n=28）和宫颈鳞状细胞癌组织（CA组，n=80）为研究样本；……