聚乙二醇胁迫对酸浆幼苗生理特性的影响
2018-06-07于爽李帅高剑郝婧玮才忠喜
于爽 李帅 高剑 郝婧玮 才忠喜



摘要:为探明酸浆(Physali alkekengi L.)幼苗对不同干旱处理的生理响应机制,为耐旱酸浆资源的开发利用提供科学依据,以酸浆的两个品种粒粒甜和铁把幼苗为材料,采用PEG-6000(质量分数0、5%、10%、15%、20%、30%)模拟干旱胁迫的方法,研究在干旱条件下酸浆两个品种幼苗的生理生化特性。结果表明,随着PEG-6000浓度升高,丙二醛(MDA)含量呈现持续上升趋势,超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、脯氨酸(Pro)含量和可溶性糖(SS)含量呈现先上升再下降的趋势。粒粒甜的POD、CAT活性以及可溶性糖含量在15%的PEG-6000处理下达到最高值,而铁把的POD、CAT活性以及可溶性糖含量分别在20%、15%和10%的浓度下达到最高值。在PEG-6000浓度20%时,两个品种酸浆幼苗的SOD活性和Pro含量均达到最大值。粒粒甜对PEG-6000的耐受性好于铁把。
关键词:酸浆(Physali alkekengi L.);PEG-6000;生理特性
中图分类号:Q949.777.7 文献标识码:A 文章编号:0439-8114(2018)07-0087-04
DOI:10.14088/j.cnki.issn0439-8114.2018.07.020
Effects of PEG Stress on Physiological Characteristics of Seedlings of
Physali alkekengi L.
YU Shuang, LI Shuai, GAO Jian, HAO Jing-wei, CAI Zhong-xi
(College of Life Science and Technology, Mudanjiang Normal College, Mudanjiang 157011, Heilongjiang, China)
Abstract: In order to explore the physiological response mechanism of Physali alkekengi L. in different drought treatments,and provide scientific references for exploration and utilization of drought tolerant varieties, Lilitian and Tieba seedlings were used as materials. The physiological and biochemical characteristics of two kinds of Physalis were measured by PEG-6000(mass fraction 0,5%,10%,15%,20%,30%) simulation drought stress. The results showed that with the increasing concentration of PEG-6000,malondialdehyde(MDA) content showed a rising trend,superoxide dismutase(SOD) activity, peroxidase(POD) activity,catalase(CAT) activity,proline(Pro) content and soluble sugar content(SS) first increased and then decreased. The POD,CAT activity and soluble sugar content of Lilitian reached the highest value under the PEG-6000 treatment of 15% concentration,while the POD,CAT activity and soluble sugar content of Tieba reached the highest value at 20%,15% and 10% concentrations respectively. PEG-6000 concentration arrive at 20%,SOD activity and Pro content of two varieties of seedlings reached the maximum. Lilitian is better tolerated to PEG-6000 than Tieba.
Key words: Physali alkekengi L.; PEG-6000; physiological characteristics
干旱是自然界最严重的灾害之一,在中国发生频率连年增长,严重制约了植物的生长、发育和产量。聚乙二醇PEG-6000作为一种渗透调节剂,可以模拟干旱胁迫,被应用于研究种子的萌发以及幼苗的生长[1,2]。近年来,许多科学研究工作者对黄瓜、油菜、番茄等耐旱性做出了大量研究[3-5]。
酸浆(Physali alkekengi L.)为茄科酸浆属多年生草本植物,具有较高的食用、药用和观赏价值。酸浆是中国民间很受喜爱的水果,味道酸甜,富含维生素C,此外,含有甾醇、黄酮、多糖等活性成分[6,7]。酸浆在国内广泛分布,但栽培主要在东北和华北地区。目前,有关酸浆深加工的研究已有报道,如酸浆复合饮料和发酵酒的研发,酸浆果脯、果酱的加工等[8]。……
