顾其芳 张红芝 朱颖莹 鲁珺琰 於颖 刘诚 刘玥 陈敏

摘要：【目的】比较三种金黄色葡萄球菌选择性分离培养基的检测效果。【方法】分别使用Baird-Parker培养基（BP）、兔血浆纤维蛋白原培养基（RPF）和科玛嘉葡萄球菌显色培养基（CHROMagarTMStaph aureus）对标准菌株、食品分离株以及食品样品进行对比检测。【结果】三种选择性培养基对金黄色葡萄球菌的回收率均达到95%以上；RPF和CHROMagarTMStaph aureus培养基的检测效率明显高于BP培养基；且两种培养基敏感性、特异性均优于传统的BP培养基。【结论】应用RPF和CHROMagarTMStaph aureus培养基检测食品中金黄色葡萄球菌，较传统的BP培养基具有更好的检测效果，建议将RPF和CHROMagarTMStaph aureus培养基作为可靠的分离检测培养基应用于食品中金黄色葡萄球菌的分离检测。

关键词：金黄色葡萄球菌；Baird-Parker培养基；兔血浆纤维蛋白原培养基（RPF）；科玛嘉葡萄球菌显色培养基；检测效果

中图分类号：R195 文献标志码：A DOI：10.19428/j.cnki.sjpm.2018.18378

引用格式：顾其芳，张红芝，朱颖莹，等.三种金黄色葡萄球菌选择性分离培养基的检测效果比较[J].上海预防医学，2018，30（1）：69-73.

Detection effects compared between three types of selective media for Staphylococcus aureus GU Qi-fang，ZHANG Hong-zhi，ZHU Ying-ying，LU Jun-yan，YU Ying，LIU Cheng，LIU Yue，CHEN Min（Shanghai Municipal Center for Disease Control and Prevention，Shanghai 200336，China）

Abstract： [Objective] To compare the detection effects between three kinds of selective media for Staphylococcus aureus. [Methods] Baird-Parker （BP） agar，Rabbit Plasma Fibrinogen （RPF） agar and CHROMagarTMStaph aureus were selected to test the standard strains，food-isolated strains as well as food samples. [Results] More than 95% recovery efficiency could be reached by utilization of all the three types of media. However，the detection effects of RPF and CHROMagarTMStaph aureus were significantly higher than that of BP agar.Moreover，their sensitivity and specificity were also superior to that of conditional BP agar. [Conclusion] Better detection effect is obtained from RPF and CHROMagarTMStaph aureus when compared to that from BP agar，suggesting that RPF and CHROMagarTMStaph aureus agar are more reliable for the detection of Staphylococcus aureus in food.

Keywords：Staphylococcus aureus；Baird-Parker agar；Rabbit Plasma Fibrinogen agar（RPF）；CHROMagarTMStaph aureus；detection effect

金黃色葡萄球菌是引起人类食源性疾病的重要病原菌之一，对其的微生物检测方法目前仍以常规分离培养为主。GB 4789.10—2016《食品安全国家标准 食品微生物学检验 金黄色葡萄球菌检验》规定Baird-Parker培养基（BP）为唯一使用的选择性培养基[1]。它也是目前国内实验室最多采用的分离培养基，但此培养基不能检测出对卵黄反应阴性的金黄色葡萄球菌，其特异性不高。因此需要补充包括革兰氏染色、镜检、凝固酶等试验才能确认[2]。而……