徐德峰++马忠华++赵谋明++张婷++苏国万++段军毅

[摘要]目的：評价四种食源性肽口服暴露对SD大鼠光老化皮肤皱纹及屏障功能的改善效果。方法：将112只健康成年雌性SD大鼠随机平均分为空白对照组、模型组、肽样品组，每种肽分别设定低、中、高剂量组，配制浓度为0.3、0.9、1.5g/L的含肽水溶液进行口服，每日自由饮用；空白组不施行紫外辐照，模型组及肽样品组均行UVA+UVB联合辐照，连续辐照18周后进行皮肤表观皱纹评分，检测皮肤表皮水分含量和油脂含量，评价4种食源性肽对光老化大鼠皮肤皱纹和屏障功能的改善效应。结果：与正常对照组相比，模型组大鼠皮肤皱纹感官质量显著降低，肽口服干预后可不同程度改善皮肤皱纹状况，且总体呈剂量-效应关系，不同活性肽之间改善效果差异较大，以样品2高剂量组效果最好；从屏障功能来看，模型组大鼠皮肤水分和油脂含量显著低于空白组（P<0.05）；4种活性肽口服干预后光老化皮肤水油屏障功能恢复良好，样品2和核桃肽中、高剂量时可显著提升表皮水分和油脂含量（P<0.05）。结论：样品2能显著减少光老化大鼠皮肤深大皱纹量，改善受损皮肤屏障功能，是口服抗皱活性肽产品开发的首选基料。

[关键词]活性肽；口服；SD大鼠；光老化；屏障功能

[中图分类号]R758.14 [文献标志码]A [文章编号]1008-6455（2017）09-0049-05

Comparative Analysis of Four Kinds of Food-derived Peptides with Oral Supplementation on the Wrinkling and Barrier Function Status in Photoaging

Skin of SD Rat

XU De-feng1，2，MA Zhong-hua1，ZHAO Mou-ming2，ZHANG Ting1，SU Guo-wan2，DUAN Jun-yi3

[1.Technique Center of Infinitus （China） Co. Ltd.，Guangzhou 510665，Guangdong，China； 2.College of Light Industry And Food Science Technology，South China University of Technology，Guangzhou 510640，Guangdong，China； 3.College of Agriculture and Animal Husbandry，Tibet University， Linzhi 86000， Tibet，China]

Abstract： Objective To evaluate the improvement of four kinds of food-derived peptides with oral supplementation on wrinkling and barrier function status of SD rat photoaging. Methods 112 of healthy adult female SD rats were randomly divided into normal control group， ultraviolet exposed group and peptides administration groups. Except the normal group， all animals in model and peptides administration groups were exposed to radiation of UVA in companying with UVB. The low， middle and high-dose bioactive peptides administration were obtained by preparing the water solution of peptides with concentration of 0.3， 0.9 and 1.5g/L respectively. And then the obtained peptides solution was orally administrated to the SD rats in peptides treating groups as the sole drinking source. The model and peptides groups were exposed to UV irradiation thrice weekly for 18 weeks until the photoaging model was established and then the improvement of four kinds of food-derived peptides on wrinkling and barrier function was evaluated by sensory scoring and operatically detection. Results In comparison with the rats in normal control group， the animals in model groups had remarkable wrinkling formation and this degradation in wrinkling status was improved by oral supplementation of peptides and the effectiveness was in a dose-effect manner and dependent on the kind and concentration of， of which the sample of peptides 2 exhibited most distinguished wrinkling elimination. Furtherly， judging from the water and oil barrier function of skin， the moisture and lipid concentration of skin in model group were significantly lower than those in normal group and the barrier function was desirable improved by oral supplementation of peptides （P<0.05）. At middle and high dose， the sample 2 and peptides significantly enhanced the moisture and lipid concentration of skin （P<0.05）. Conclusion Sample 2 can remarkably eliminate the deep and huge wrinkles and restore the barrier function in photoaging skin of SD rats， and thus to be the element ingredient for developing an anti-wrinkling product with peptides.endprint

Key words： peptides； oral supplementation； SD rats； photoaging； barrier function

皮肤老化与抗衰老是当今皮肤科学的研究热点，紫外线辐射引起的皮肤光老化是皮肤外源性衰老的主要原因[1]。光老化导致皮肤过早出现粗糙、皱纹、色素异常、脆性增加、毛细血管扩张及皮革样外观等症状，严重影响皮肤外观，导致皮肤屏障功能受损[2-4]。随着审美标准的不断提高，人们对皮肤光老化的防治越来越重视，鉴于皮肤组织的天然“砖墙结构”的致密屏障，只有分子量小于3 000Da以下的分子才能透皮吸收，而且功效成分大多化学结构稳定性较差，长时间暴露于紫外线等环境中极易分解，失去本身应有的抗衰功能甚至产生有毒物质[5]。……