陈椿+李舒婷+黄景珠+卢棉+梁寻杰+方晓燕+黄丽娟

【摘要】目的探究黄秋葵提取液对小鼠胃排空和小肠推进作用的影响。方法将80只小鼠随机分为黄秋葵低剂量组（1.5 g/kg）、黄秋葵中剂量组（3.0 g/kg）、黄秋葵高剂量组（6.0 g/kg）、阿托品组（5.0 mg/kg）、阿托品+黄秋葵低剂量组、阿托品+黄秋葵中剂量组、阿托品+黄秋葵高剂量组和正常对照组，每组10只小鼠。黄秋葵采用灌胃，阿托品采用肌内注射，正常对照组灌胃蒸馏水，各组按相应药液和给药方法给予相应的药物一次，采用灌碳末悬液实验方法，测量并计算小鼠胃残留率和小肠推进率。结果与正常对照组比较，黄秋葵中剂量组、高剂量组可明显促进小肠推进（P<0.05或0.01），且高剂量组小肠推进率高于低剂量组（P<0.05）；与阿托品组比较，阿托品+黄秋葵中剂量组、阿托品+黄秋葵高剂量组小肠推进率均显著升高（P<0.01），且高剂量组高于低剂量组（P<0.05），而阿托品+黄秋葵高剂量组胃残留率低于阿托品组（P<0.05）。结论黄秋葵能促进小肠运动，其机制可能与拟胆碱样作用有关。

【关键词】黄秋葵；小鼠；胃排空；小肠推进；阿托品

中图分类号：R573；R965文献标识码：ADOI：10.3969/j.issn.1003-1383.2017.04.017

【Abstract】ObjectiveTo explore the effect of okra extract on gastric emptying and intestinal propulsion in mice.Methods80 mice were randomly divided into eight groups：low dose okra group（1.5 g/kg），middle dose okra group（3 g/kg），high dose okra group（6 g/kg），atropine group（5 mg/kg），atropine+low dose okra group，atropine+middle dose okra group，atropine+high dose okra group and normal control group with 10 mice in each group.Okra was used by gastric perfusion.Atropine was used by intramuscular injection.And normal control group was given distilled water by gastric perfusion.And then each group were given corresponding medicine according to the corresponding medicine and the method of administration.By using experimental method with perfusion of carbon suspension，and gastric residual rate and intestinal propulsive rate were measured and calculated.ResultsCompared with the normal control group，the middle dose okra group and high dose okra group could obviously promote small intestine propulsion（P<0.05 or 0.01），and intestinal propulsive rate of the high dose group was significantly higher than that of the low dose group （P<0.05）.Compared with the atropine group，the intestinal propulsive rate of the atropine+middle dose okra group and the atropine+high dose okra group increased significantly（P<0.01），and that of the high dose group was significantly higher than that of the low dose group（P<0.05），while the rates of gastric remainder of atropine+high dose okra group was obviously lower than that of the atropine group （P<0.05）.ConclusionOkra can promote the intestinal movement，its mechanism may be related to the effect of choline analogues.

【Key words】okra；mice；gastric emptying；intestinal propulsion；atropine

功能性消化不良是臨床常见病，越来越受到人们重视，而胃肠动力异常是其病理生理机制之一[1]，我国中药在调理改善胃肠功能活动方面有诸多优势。黄秋葵[Abelmoschus esculentus（L.）Moench]为一年生的草本植物，别名秋葵、羊角豆等，发源于非洲，现世界各地均有栽培。其嫩果供食用，果实含有丰富的蛋白质、粗纤维、多糖、黄酮、脂肪及其他等[2]，含有丰富的常量元素，其中K+含量最高，其次是P和Ca[2～3]。其果实也含有丰富的黏液，主要由果胶和多糖等组成，能促进人体胃肠蠕动，有效防止便秘。但目前尚未发现有黄秋葵对胃肠动力影响的相关实验研究。本研究通过观察黄秋葵水提取液以及在阿托品干预作用下对小鼠小肠运动及胃排空的影响，为黄秋葵进一步开发利用提供实验依据。……