苏获，毛林锋，Benson Kaluba，段智曦，刘擎，黄鹏

（1.中南大学护理学院，湖南 长沙 410013；2.中南大学湘雅医院，湖南 长沙 410008；3.中南大学湘雅二医院，湖南 长沙 410011）

自制爬片装置用于细胞爬片及细胞染色

苏获1，毛林锋2，Benson Kaluba2，段智曦3，刘擎2，黄鹏2

（1.中南大学护理学院，湖南 长沙 410013；2.中南大学湘雅医院，湖南 长沙 410008；3.中南大学湘雅二医院，湖南 长沙 410011）

目的利用自制爬片装置改进细胞爬片的制备方法。方法通过自制装置和传统方法制备细胞爬片，苏木精-伊红染色法（HE染色）观察细胞生长状态，比较两种细胞爬片制备方法的差异；利用自制装置制备同时给予3种不同干预处理的细胞爬片，验证其应用于制备多干预条件细胞爬片；制备同时含有3种不同细胞的集成爬片，验证其用于制作细胞集成爬片的应用。结果自制装置可简化细胞爬片流程，细胞直接贴于载玻片上生长，未出现污染和增殖受限，制作多干预或多种细胞集成爬片效果明确，无相互干扰现象。结论自制装置能更简便地制备以满足普通染色及特殊染色实验要求的细胞爬片，是一种更好的制备细胞爬片的方法。

细胞爬片；细胞培养；染色

Abstract:ObjectiveTo provide an improved method to prepare cell-attached slides.MethodsCellattached slides were prepared by routine method and self-made device,and the difference between the two methods were compared by observing the cell growth after HE staining.Three kinds of interventions were given to the same cell-attached slide to test the application in making different interventions cell-attached slide of the device.Three kinds of cells were planted in the same slide to test the application in making integrated cell-attached slides of the device.ResultsOur device simplified the preparation of cell-attached slides.Cells grown on the glass and never showed pollution or growth restriction,given different interventions or planted different cells at the same time without interaction.ConclusionsThe new method simplifies the processes of preparation cell-attached slides,and the slides made by our device satisfies the experiment demands of all kinds of cells staining,makes it as a more effective method than before.

Keywords:cell-attached slide;cells culture;cells staining

细胞爬片是观察细胞形态结构最常用的一种实验手段，利用细胞爬片进行体外实验可排除体内诸多干扰因素的影响，并方便对细胞进行各种干预处理[1]。传统的方法是在装有盖玻片的孔板中种入细胞，待细胞在盖玻片上贴壁生长后再取出盖玻片进行后续的染色实验，整个过程操作起来比较繁琐，且存在许多缺陷。……