于竞新 李冰韶 邱玥 张晓阳 连增林 吴清

摘要：目的 筛选连翘、防风和辛夷混合的挥发油提取及β-环糊精包合的最佳工艺。方法 以混合挥发油收取量为指标，单因素试验考察混合挥发油的最优提取工艺；选用饱和水溶液法，以混合挥发油包合率为指标，正交试验设计考察混合挥发油与β-环糊精投料比、包合时间和包合温度对包合工艺的影响；通过X射线衍射法、红外光谱法以及扫描电镜法对包合物进行验证。结果 混合挥发油提取最优工艺为：加10倍量水，浸泡3 h，粉碎成细粉，提取5 h。最佳包合工艺为：挥发油（mL）与β-环糊精（g）比例为1∶10，包合温度为50 ℃，包合时间为2 h。X射线衍射法、红外光谱法及扫描电镜均表明混合挥发油包合物构建相对稳定。结论 优选的提取和包合工艺稳定可行，为进一步制剂开发提供了研究基础。

关键词：连翘；防风；辛夷；混合挥发油；β-环糊精；包合工艺

DOI：10.3969/j.issn.1005-5304.2017.08.018

中图分类号：R283.5 文献标识码：A 文章编号：1005-5304（2017）08-0080-05

Study on Extraction and Inclusion Processes of Mixed Volatile Oils from Forsythiae Fructus， Saposhnikoviae Radix and Magnoliae Flos YU Jing-xin1， LI Bing-shao1， QIU Yue1， ZHANG Xiao-yang2， LIAN Zeng-lin3， WU Qing1 （1. School of Chinese Materia Medica， Beijing University of Chinese Medicine， Beijing 100102， China； 2. TCM Department， Peking Union Medical College Hospital， Beijing 100730， China； 3. TCM Biotechnology Institute of Beijing Yichuang Biotechnology Academy， Beijing 101111， China）

Abstract： Objective To optimize the extraction process of mixed volatile oil from Forsythiae Fructus， Saposhnikoviae Radix and Magnoliae Flos and inclusion process of β-cyclodextrin （β-CD）. Methods With yield ratio of volatile oil as evaluation index， single factor experiments were used to study the extraction process of volatile oil； saturated aqueous solution was used， with inclusion rate of volatile oil as index， and orthogonal design was adopted to examine effects of charge ratio of volatile oil and β-CD， inclusion temperature and inclusion time on the inclusion process； X-ray scattering technique， infrared spectroscopy and scanning electron microscopy were used to characterize the inclusion compound. Results The optimum extraction process of volatile oil was soaking fine powder extracted 5 hours with 10 folds the amount of water. The optimum conditions of inclusion process were as follows： mixed ratio of volatile oil （mL） and β-CD （g） was 1：10； inclusion temperature was 50 ℃； the inclusion time was 2 h. X-ray scattering technique， infrared spectroscopy and scanning electron microscopy proved the inclusion compound had been formed. Conclusion Optimum extraction and inclusion processes are stable and feasible， and can provide research foundation for further research and development of preparation.

Key words： Forsythiae Fructus； Saposhnikoviae Radix； Magnoliae Flos； mixed volatile oil； β-cyclodextrin； inclusion process

祛風胜湿方是北京协和医院中医科治疗变应性鼻炎的临床经验方，其基于中医风药理论，以祛风、疏风、散风药物为主，辅以开窍清解之品立法配伍而成。全方温清并举、疏风散邪、祛风胜湿，对变应性鼻炎具有良好的治疗作用。该方由连翘、防风和辛夷

通讯作者：吳清，E-mail：qwu@vip.sina.com

等药组成，连翘、防风和辛夷均含挥发油且具有抗炎、抗过敏作用。荆防挥发油对大鼠弗氏完全佐剂所致的关节炎肿胀、小鼠被动异种皮肤过敏反应抑制作用明显[1]。辛夷挥发油能够调节变态反应性鼻炎豚鼠外周血中Th细胞免疫，从而维持Th1/Th2的动态平衡[2]。……