唐颖楠 彭颖 唐林 周莉莉 夏新华

摘要：目的 优选清解颗粒挥发油的提取和β-环糊精包合工艺。方法 采用水蒸馏法进行提取，以提取时间、粉碎度、加水倍数为考察因素，挥发油体积为评价指标，考察清解颗粒挥发油的提取工艺；以包合率为评价指标，采用单因素试验和Box-Behnken响应面法结合，优选最佳包合工艺。结果 优选的提取工艺为荆芥、防风、连翘药材粗粉加10倍量水，提取3 h。包合方法为饱和水溶液法，包合工艺为挥发油与β-环糊精的投料比为1∶12、包合温度40 ℃、包合时间3.5 h。通过薄层色谱法、紫外光谱法和红外光谱法初步证明了清解颗粒挥发油包合物的生成。结论 优化的清解颗粒挥发油提取和包合工艺稳定可行，可用于工业化大生产。

关键词：挥发油；Box-Behnken设计；β-环糊精包合物；提取工艺；包合工艺

DOI：10.3969/j.issn.1005-5304.2017.08.016

中图分类号：R283.5 文献标识码：A 文章编号：1005-5304（2017）08-0070-06

Extraction Technology of Volatile Oil from Qingjie Granules and Optimization of Inclusion Technology by Box-Behnken TANG Ying-nan1， PENG Ying1， TANG Lin2， ZHOU Li-li1， XIA Xin-hua1 （1. School of Pharmacy， Hunan University of Chinese Medicine， Changsha 410208， China； 2. The First Hospital of Hunan University of Chinese Medicine， Changsha 410007， China）

Abstract： Objective To optimize the extraction of volatile oil from Qingjie Granules and process of inclusion compound of beta-cyclodextrin. Methods Water Distillation was used for extraction. Extraction time， grinding degree， and the amount of water were set as inspection factors， and volatile oil volume was set as the evaluation index to inspect extraction process of volatile oil from Qingjie Granules. With inclusion rate as the evaluation index， the single factor test and the Box-Behnken combined with the response surface method were used to choose the optimum inclusion process. Results The optimum extraction process for Schizonepetae Herba， Saposhnikoviae Radix and Forsythiae Fructus coarse powder should be with 10 times amount of water， extracting 3 h. Inclusion method should be saturated water solution method， and the inclusion process of volatile oil as feed and beta-cyclodextrin inclusion ratio was 1：12； the temperature was 40 ℃； inclusion time was 3.5 h. By means of TLC， UV and IR spectra， the formation of the inclusion compound of volatile oil in clear solution particles was preliminarily proved. Conclusion The optimum extraction and inclusion process of volatile oil from Qingjie Granules are stable and feasible， which can be used in industrial production.

Key words： volatile oil； Box-Behnken design； beta-cyclodextrin inclusion compound； extraction process； inclusion process

清解顆粒为湖南中医药大学附属第一医院治疗急、慢性支气管炎的临床经验方，由荆芥、防风、连翘、苦杏仁、桔梗等12味药组成，其中荆芥、防风、连翘（青翘）含有较多的挥发油，与其解热抗炎、祛痰平喘的药理作用关系密切[1]。为减少挥发油成分对胃肠道黏膜的刺激性，提高药物的稳定性，保证药物质量，我们选用β-环糊精对清解颗粒中荆芥、防风、连翘混合挥发油进行包合，以便开发为现代中成药制

通讯作者：夏新华，E-mail：xiaxinhua001@163.com

剂。本试验采用水蒸馏法考察清解颗粒挥发油提取工艺，并采用饱和水溶液法，单因素试验结合Box-Behnken响应面法，考察影响包合工艺的主要因素，优化清解颗粒挥发油包合工艺。……