吴道勋 张娜 邵维莉 杨贤英 陈进汝 刘熙

摘要：目的 觀察云南松松塔提取物对小鼠急性酒精性肝损伤的影响，探讨其作用机制。方法 采用酒精灌胃制备急性酒精性肝损伤小鼠模型。实验小鼠随机分为正常组、模型组、阳性对照组和云南松松塔低、中、高剂量组，各给药组给予相应药物灌胃，每日1次，连续7 d。末次灌胃3 h后，计算肝、脾指数，检测丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、丙二醛（MDA）、一氧化氮（NO）含量，超氧化物歧化酶（SOD）、微量还原型谷胱甘肽（GSH）活性，HE染色观察肝脏病理形态。结果 与模型组比较，云南松松塔各剂量组小鼠肝指数显著降低（P<0.01），云南松松塔高剂量组脾指数显著降低（P<0.01），云南松松塔中、高剂量组血清AST含量显著降低（P<0.01），云南松松塔各剂量组肝组织GSH活性显著升高（P<0.05，P<0.01）；云南松松塔低、高剂量组肝组织MDA含量显著降低（P<0.05）；云南松松塔各剂量组血清ALT含量降低，肝组织SOD活性升高和NO含量降低，差异均无统计学意义（P>0.05）；HE染色结果显示，云南松松塔各剂量组小鼠肝组织损伤较模型组明显改善。结论 云南松松塔提取物对小鼠急性酒精性肝损伤具有保护作用。

关键词：云南松松塔提取物；急性酒精性肝损伤；氧化因子；小鼠

DOI：10.3969/j.issn.1005-5304.2017.08.011

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）08-0046-04

Effects of Pinus Yunnanensis on Acute Alcoholic Liver Injury in Mice WU Dao-xun1， ZHANG Na1， SHAO Wei-li1， YANG Xian-ying1， CHEN Jin-ru1， LIU Xi1，2 （1. College of Pharmacy and Chemistry， Dali University， Dali 671000， China； 2. Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D;， Dali 671000， China）

Abstract： Objective To study the effect of Pinus yunnanensis on acute alcoholic liver injury in rats and explore its mechanism. Methods A model of acute alcoholic liver injury in mice was prepared by alcohol. The mice were randomly divided into normal control group， model group， positive control group， Pinus yunnanensis low-， medium- and high-dose groups. Mice in the medicine group were given the corresponding medicine by gavage once a day for 7 days. After the last three hours of intragastric administration， the liver and spleen index， ALT， AST and GSH in serum， SOD， MDA and NO in liver homogenates were measured. Histopathological changes of liver were observed by HE staining. Results Compared with model group， Pinecone of Pinus unnanensis high-， medium- and low-dose groups could significantly reduce the liver index in mice （P<0.01）， and high dose groups could significantly reduce the number of spleen （P<0.01）； The contents of AST in the medium- and high-dose groups significantly decreased （P<0.01） and the GSH activity significantly increased （P<0.05， P<0.01）. There was no significant difference in serum ALT level， SOD activity， GSH activity and NO content in the liver tissues of Pinus yunnanensis groups （P>0.05）. HE staining results showed that， the damage of liver tissue in mice of Pinus yunnanensis was significantly improved compared with the model group. Conclusion Pinus yunnanensis has protective effects on acute alcoholic liver injury in mice.

Key words： Pinus yunnanensis； acute alcoholic liver injury； oxidation factors； mice

酒精性肝病（alcoholic liver disease，ALD）是酗

基金项目：国家自然科学基金（81260632）

通讯作者：刘熙，E-mail：626585322@qq.com

酒导致的肝脏损害性疾病，在病理组织学上按照肝细胞发生脂肪病变的程度分为酒精性脂肪肝、酒精性肝炎、酒精性肝纤维化及酒精性肝硬化。ALD是一种进行性发展且后期严重危害身体健康和威胁生命安全的疾病，其致病因素单一、明确，即长期大量的饮酒所致，但发病机制十分复杂，至今尚未完全明了，目前治疗手段主要是戒酒、营养支持和药物治疗。松塔（Pinecone）系松科（Pinaceae）松属（Pinus）植物的球果，别名松实、松元、松果、松球。……