张宇

[摘要] 目的 探讨参草通脉颗粒对慢性心衰大鼠MMP-3与TIMP-3的影响。方法 随机选取10只大鼠作为正常对照组，10只作为假手术组，40只构建慢性心衰模型，建模成功后随即选取30只，分为模型组、西药组及中药组，各10只；对照组、假手术组及模型组采用蒸馏水灌胃，西药组给予赖诺普利处理，中药组给予参草通脉颗粒处理；Western blot法检测心肌组织中MMP-3、TIMP-3蛋白表達量。结果 与正常对照组相比，假手术组MMP-3表达有所增加，TIMP-3表达有所下降，但组间比较差异不显著（P>0.05）；与假手术组相比，模型组MMP-3表达显著增加，TIMP-3表达显著下降（P<0.05）；中药组及西药组与模型组相比，MMP-3表达显著下调且TIMP-3表达显著上调（P<0.05），但并未恢复到假手术组水平；中药组与西药组组间比较MMP-3及TIMP-3表达差异不显著（P>0.05）。此外，中药组、西药组与模型组相比，LVIDs、LVIDd下降，EF 值上升（P<0.05）。结论 参草通脉颗粒可以下调MMP-3的表达，增加TIMP-3的表达，从而阻止MMP-3对心肌细胞外基质结构的破坏作用，阻止CHF的发展。

[关键词]参草通脉颗粒；心力衰竭；MMP-3；TIMP-3

[中图分类号] R-332 [文献标识码] A [文章编号] 2095-0616（2017）11-33-04

Experimental study on effects of Shencao Tongmai granule on MMP-3 and TIMP-3 in chronic heart failure rats

ZHANG Yu

Liaoning University of Traditional Chinese Medicine， Liaoning 110032， China

[Abstract] Objective To explore the effects of Shencao Tongmai granule on MMP-3 and TIMP3 expressions in chronic heart failure rats. Methods 10 rats were randomly selected as control group， other 10 as sham-operated group， and 40 rats were chosen underwent chronic heart failure model building， within which 30 were chosen as model group（10 cases）， western medicine group（10 cases）， and traditional Chinese medicine group（10 cases）， randomly. The control group， sham-operated group and model group were given gastric perfusion of distilled water. Western medicine group was taken lisinopril and the traditional Chinese medicine group was taken Shencao Tongmai granule. The expressing quantities of MMP-3 and TIMP3 in myocardial tissue were detected by Western blot. Results Compared with control group， the expression of MMP-3 in sham-operated group was increased and TIMP-3 decreased（P>0.05）. Compared with sham-operated group， the expression of MMP-3 in model group was increased and TIMP-3 decreased（P<0.05）. Compared with western medicine group and traditional Chinese medicine group， the expression of MMP-3 was decreased and TIMP-3 increased（P<0.05）. There was no significant difference of MMP-3 and TIMP-3 between western medicine group and traditional Chinese medicine group（P>0.05）. In addition， compared with model group， LVIDs and LVIDd decreased and EF values increasedin the traditional Chinese medicine group and western medicine group（P<0.05）. Conclusion Shencao Tongmai granule can reduce the expression of MMP-3 and promote the expression of TIMP-3， so as to stop the destruction of MMP-3 to myocardial extracellular matrix and to impede the progress of CHF.

[Key words] Shencao Tongmai granule； Cardiac failure； MMP-3； TIMP-3

慢性心力衰竭是指心脏的泵血功能不足，导致各组织灌注不足从而发生肺循环或（和）体循环淤血，是临床各种心脏疾病的最终发展[1-2]。细胞外基质（extracellular matrix，EMC）的正常合成与降解是心脏維持正常生理功能的基础；有研究表明，EMC的重构在慢性心力衰竭疾病的发生发展过程中起重要作用，而EMC的重构是体内基质金属蛋白酶（matrix metalloproteinase，MMPs）及其组织抑制因子（tissue inhibitor of metalloproteinase，TIMPs）动态平衡的结果[3-4]。参草通脉颗粒是我院自行研制的中药制剂，临床用于治疗慢性心力衰竭且取得较好疗效[5]。基于此等理论基础，本研究探讨了经参草通脉颗粒处理后，慢性心力衰竭大鼠心肌基质金属蛋白酶-3（matrix metalloproteinase 3，MMP-3）和基质金属蛋白酶特异性抑制物-3（tissue inhibitor of metalloproteinase-3，TIMP-3）表达的变化，旨在了解该病的致病机理并未该病治疗提供新的作用靶位点。