梁倩++++++唐伟++++++刘文博++++++王威

[摘要] 目的 研究大鼠腦β-淀粉样蛋白表达及神经行为学与高胆固醇水平的关系，以明确辛伐他汀对阿尔茨海默病（AD）防治有一定作用。 方法 将32只健康Wistar大鼠按随机数字表法分为普通组和高脂组，每组16只。饲养2周后，再将普通组随机分为普通药物组和普通安慰剂组，每组8只；将高脂组大鼠随机分为高脂药物组、高脂安慰剂组，每组8只。药物组16只大鼠给予辛伐他汀灌胃，安慰剂组给同体积安慰剂（蒸馏水）灌胃。免疫组织化学法测定脑内β-淀粉样蛋白（Aβ）蛋白的表达，Morris水迷宫实验测试大鼠的学习和记忆能力。 结果 高脂药物组大鼠前额皮层Aβ1-42的蛋白表达较高脂安慰剂组减少（P < 0.05）；高脂药物组与普通安慰剂组、普通药物组之间比较，差异无统计学意义（P > 0.05）。与高脂安慰剂组比较，高脂药物组大鼠逃避潜伏期缩短（P < 0.05），穿台次数增多（P < 0.05）；高脂药物组与普通安慰剂组、普通药物组之间比较，差异无统计学意义（P > 0.05）。 结论 辛伐他汀对高胆固醇血症大鼠前额皮层Aβ1-42的蛋白表达有一定影响，同时伴随大鼠神经行为学改变。

[关键词] 高胆固醇；辛伐他汀；β-淀粉样蛋白；神经行为学

[中图分类号] R74 [文献标识码] A [文章编号] 1673-7210（2017）06（a）-0024-04

The relationship between the expression of brain amyloid beta protein along with neural behavior in rats with hypercholesterolemia level

LIANG Qian1 TANG Wei2 LIU Wenbo1 WANG Wei1

1.Department of Neurology， Affiliated Zhongshan Hospital of Dalian University， Liaoning Province， Dalian 116001， China； 2.Department of Neurology， Affiliated Xinhua Hospital of Dalian University， Liaoning Province， Dalian 116021，China

[Abstract] Objective To research the relationship between the expression of brain amyloid beta （Aβ） protein along with neural behavior in rats with hypercholesterolemia level， in order to clarify the preventive effect of simvastatin on Alzheimer's disease. Methods The 32 Wistar rats were divided into normal group and high cholesterol group according to random number table， with 16 rats in each group. After two weeks， the rats in the normal group were randomly divided into normal drug group and normal placebo group， with 8 rats in each group； and the rats in the high cholesterol group were randomly divided into high fat drug group and high fat placebo group， with 8 rats in each group. And then simvastatin were filled into the stomach of the 16 drug group rats， the same volume placebo （distilled water） were filled into the stomach of placebo group. Immunohistochemical method was used to determine the expression of Aβ protein in brain， Morris water maze was used to test the cognitive and memory abilities in rats. Results Aβ1-42 protein expression of the prefrontal cortex in the high fat drug group was less than that of high fat placebo group （P < 0.05）； and there was no significant difference in the high fat drug group， normal placebo group and normal drug group （P > 0.05）. In the high fat drug group， the escape latency was decreased than that of high fat placebo group （P < 0.05）， times of crossing platform was increased （P < 0.05）； and there was no significant difference in the high fat drug group， normal placebo group and normal drug group （P > 0.05）. Conclusion Simvastatin has some effects on the expression of Aβ1-42 protein in prefrontal cortex of hypercholesterolemic rats， and accompanied by neurobehavioral changes in rats.

[Key words] High cholesterol； Simvastatin； Beta amyloid protein； Neural behavior

阿尔茨海默病（Alzheimer′s disease，AD）是常见的中枢神经系统变性疾病，主要表现为进行性记忆减退和认知功能障碍，据流行病学统计，全世界至少有2000～3000万AD患者[1]。虽然近年来AD的研究取得较大的进展，但AD的病因迄今未明，目前对AD的治疗没有明确有效的措施。AD的危险因素除了遗传，年龄增长，载脂蛋白E基因型以外，糖尿病，高热量的饮食，血脂异常及吸烟与AD的发生有一定关系[2]。AD的主要病理改变是老年斑的形成，神经元纤维缠结以及广泛神经元缺失。……