胡玉涛 李天雪 苟琼心

摘要 [目的]建立同时测定盐碱地种植的红白忍冬和金银花中新绿原酸、绿原酸、咖啡酸、木犀草苷和木犀草素的HPLC方法，并比较不同植物器官中5种成分的含量。[方法]色谱柱为Inertsil ODS-SP C18 柱（250 mm×4.6 mm，5 μm）；流动相为0.1%甲酸水溶液-乙腈；体积流量1.0 mL/min；检测波长327 nm；柱温30 ℃。[结果]新绿原酸、绿原酸在1.0～500.0 μg/mL，咖啡酸在0.2～100.0 μg/mL，木犀草苷、木犀草素在0.1～50.0 μg/mL具有良好的线性关系；5种成分的日内和日间精密度RSD值均≤4.2%，且在36 h 内稳定，平均回收率为95.60%～97.35%。红白忍冬和金银花药材样品中5种化合物的含量差异较显著，在各器官中金银花5种化合物均比红白忍冬高，但红白忍冬中5种化合物的含量亦有较高水平。[结论]该方法简单便捷，适合于红白忍冬中生物活性化合物的定量测定研究，能为该药材的质量控制制定提供科学、可靠的分析技术。

关键词 红白忍冬；金银花；新绿原酸；绿原酸；咖啡酸；木犀草苷；木犀草素；高效液相色谱；定量分析

中图分类号 R284 文献标识码 A 文章编号 0517-6611（2017）34-0126-02

Abstract [Objective] The research aimed to establish HPLC method for simultaneous determination of neochlorogenic acid，chlorogenic acid，cafferic acid，luteoloside and luteolin in L.japonica var.and L.japonica Thunb.cultivated in saline soil，and compare the contents of five components in different plant organs.[Method]The chromatographic column was Inertsil ODSSP C18 column （250 mm × 4.6 mm，5 μm）； the mobile phase was 0.1% formic acid aqueous solutionacetonitrile；volume flow rate was 1.0 mL/min； detection wavelength was 327 nm； column temperature was 30 ℃.[Result]Neochlorogenic acid and chlorogenic acid in 1.0 -500.0 μg/mL，caffeic acid in 0.2 - 100.0 μg/mL，luteoloside and luteolin in 0.1 - 50.0 μg/mL had a good linear relationship；The RSD values of the inter- and intra- precisions were less than 4.2%.The stability was accepted within 36 h.The average recovery rate were 95.60% -97.35%.The contents of five compounds in L.japonica Thunb.were higher than those in L.japonica var.，but the contents of five compounds in L.japonica var.was enough.[Conclusion]The method is simple and convenient，and is suitable for the quantitative determination of biologically active compounds in Lonicera japonica var..It can provide scientific and reliable analytical techniques for the quality control of the medicinal materials.

Key words Lonicera japonica var.；Lonicera japonica Thunb.； Neochlorogenic acid； Chlorogenic acid； Cafferic acid；Luteoloside；Luteolin； HPLC；Quantification

紅白忍冬[Lonicera japonica var.chinensis （Wats.） Bak.]为忍冬科忍冬属，半常绿缠绕木质藤木，是忍冬科植物忍冬的一个变种[1]，其枝蔓呈红色、叶淡紫色，花随花期不同，呈红色、白色、黄色等颜色，香味浓，集药用、观赏于一体，已在全国大面积推广种植。红白忍冬能更为快速地响应盐胁迫，通过提高抗氧化酶活性，增强耐盐性，维持正常的生长发育[2]。《中国药典》2015年版及相关文献对红白忍冬或金银花的质量控制主要为采用HPLC测定绿原酸和木犀草苷的含量[3-4]，药理研究表明新绿原酸和异绿原酸同绿原酸均具有抗菌、抗病毒活性[5]。……