稻米中超氧化物歧化酶含量及活性分析
2017-07-13杨先炯
杨先炯

摘要[目的]对不同稻米中超氧化物歧化酶(SOD)的含量及活性进行测定、分析。[方法]采用沉淀法从不同种类稻米中提取SOD,然后用改良的邻苯三酚自氧化法进行SOD活性分析,比较不同稻米中SOD含量及活性的差异。[结果]糯米中的SOD含量高于粳米,但两者活性差异不大。高纬度地区所产稻米的SOD含量及活性均高于低纬度地区所产稻米。胚芽保留程度高的鲜糙米SOD含量及活性最高。[结论]沉淀法可以有效地从稻米中提取SOD。不同种类、产区和加工工艺稻米中SOD含量及活性存在差异。
关键词 稻米;超氧化物歧化酶;含量;活性;邻苯三酚自氧化
中图分类号 S511 文献标识码 A 文章编号 0517-6611(2017)02-0011-02
Abstract[Objective] To determine and analyze the content and activity of superoxide dismutase (SOD) in different types of rice.[Method] SOD was isolated by precipitation process from different types of rice, and its activity was determined through modified pyrogallol selfoxidation.[Result] Glutinous rice had higher SOD content than japonica rice, however, SOD activity did not have significant difference. Both of SOD content and activity in rice from high latitude area were higher than that of rice from low latitude region. Moreover, brown rice with germ exhibited the highest SOD content and activity.[Conclusion] Precipitation process is an effective method for SOD isolation from rice. There are significant differences of SOD content and activity in different types of rice.
Key words Rice;Superoxide dismutase;Content;Activity;Pyrogallol selfoxidation
超氧化物歧化酶(SOD)是生物体内的重要抗氧化酶,具有特殊的生物学活性[1]。
SOD通过专一催化超氧阴离子自由基产生歧化反应而特异清除超氧阴离子,从而防御生物体内氧中毒,保护机体免受氧化损伤,是生物体内清除自由基的重要物质。人们已对SOD的化学结构、生物活性、药理作用及临床应用等进行了系列研究,证实SOD与机体衰老、肿瘤发生、自身免疫性疾病和辐射防护等关系密切,是一种很有临床价值的治疗酶[2]。目前,SOD 的研究越来越受到人們的重视,在食品工业、日化工业以及医药业等方面得到了广泛应用[3]。
SOD在生物界中广泛分布,以前SOD大多是从动物血、动物组织和微生物中提取[4]。近年来,人们发现植物体也含有丰富的SOD,用以清除植物正常代谢过程或环境胁迫下产生的活性氧和自由基,使植物细胞结构和功能不受氧化损伤、破坏[5]。……
