任婷 饶春梅 成细华 杨胜辉 孙彦波 陈聪 彭霞 黄政德

摘要：目的 观察加味丹参饮对SD大鼠心肌缺血再灌注损伤（IRI）模型精脒/精胺乙酰转移酶（SSAT）/多胺通路的影响，探讨其抗IRI的机制。方法 结扎大鼠冠状动脉左前降支30 min再灌注90 min建立大鼠心肌IRI模型。实验大鼠随机分为正常对照组、假手术组、模型组和加味丹参饮组，每组10只。TTC染色测定大鼠心肌梗死面积，ELISA检测大鼠心肌组织SSAT含量，RT-PCR和Western blot分别检测大鼠心肌组织SSAT mRNA和蛋白表达，HPLC检测大鼠心肌组织多胺（腐胺、精脒、精胺）含量。结果 与假手术组比较，模型组大鼠心肌梗死面积、SSAT含量增加，SSAT mRNA和蛋白表达升高，多胺含量降低（P<0.05，P<0.01）；与模型组比较，加味丹参饮组大鼠心肌梗死面积、SSAT含量减少，SSAT mRNA和蛋白表达降低，多胺含量升高（P<0.05，P<0.01）。结论 加味丹参饮可能通过调节SSAT/多胺通路，增加心肌细胞多胺含量，从而对大鼠心肌IRI发挥保护作用。

关键词：加味丹参饮；心肌缺血再灌注损伤；多胺；精脒/精胺乙酰转移酶；大鼠

DOI：10.3969/j.issn.1005-5304.2017.07.015

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）07-0062-04

Abstract： Objective To observe the effects of modified Danshen Decoction on spermidine/spermine acetyltransferase （SSAT） /polyamine pathways of SD rats with IRI； To investigate its protective mechanism. Methods The model of IRI was established by ligating left anterior descending coronary artery for 30 min followed by reperfusion for 90 min. The SD rats were randomly divided into the control group， sham-operation group， model group and modified Danshen Decoction group， with 10 rats in each group. The myocardial infarction size was measured by using TTC staining. The contents of SSAT were measured by ELISA. The SSAT mRNA and SSAT protein expression level were detected with real-time fluorescent quantitative PCR method and Western blot， respectively. The contents of polyamines （putrescine， spermidine， spermine） in cardiac tissue were detected by HPLC. Results Compared with sham-operation group， the myocardial infarction size， the SSAT content， the SSAT mRNA and SSAT protein expression levels of model group increased significantly， the contents of polyamines decreased significantly， with statistical significance （P<0.01）； Compared with model group， the myocardial infarction size of modified Danshen Decoction group was significantly reduced， while the SSAT content and SSAT mRNA and protein expression level decreased significantly， the contents of polyamines increased， with statistical significance （P<0.05， P<0.01）. Conclusion Modified Danshen Decoction can adjust the SSAT polyamine pathways and increase polyamine content in cardiomyocytes， and thus play a role of protection of myocardial ischemia-reperfusion injury.

Key words： modified Danshen Decoction； myocardial ischemia/reperfusion injury； polyamines； spermidine/ spermine acetyltransferase； rats

近年來，随着血运重建技术的广泛开展，心肌缺血再灌注损伤（ischemia reperfusion injury，IRI）机制及心肌保护的研究成为倍受重视的课题，但IRI所涉及机制复杂，目前尚未清楚，临床上仍缺乏针对心肌保护的药物。多胺（腐胺、精脒和精胺）是存在于真核细胞且含高密度正电荷的一类有机小分子，广泛参与胚胎发育、细胞生长与分化、细胞程序性凋亡及基因表达调控等重要的生理病理过程[1]。研究发现，多胺系统在IRI发挥重要作用[2]。在IRI时，多胺代谢失衡，多胺分解代谢的限速酶——精脒/精胺乙酰转移酶（spermidine/spermine N1-acetyltransferase，SSAT）表达改变，腐胺生成增多而精胺、精脒减少[3-4]，均与IRI密切相关。本课题组前期研究发现，益气活血中药复方加味丹参饮对防治心肌IRI有重要作用[5-7]。……