田会玲 李小黎 唐启盛 李侠 李庆盟 高誉珊 张雯 孙新月 邵珺

摘要：目的 探讨颐脑解郁复方对脑卒中后抑郁（PSD）大鼠行为学和海马CA1区病理损伤的干预作用。方法 将168只SPF级SD大鼠随机分为正常组、假手术组、卒中组、PSD组、西药组和中药组，正常组、假手术组各24只，其余组各30只。正常组不干预，假手术组不插入线栓，卒中组仅行大脑中动脉阻塞手术，PSD组、西药组、中药组卒中术后1周应用慢性束缚应激1周复合单笼饲养法制备PSD模型。造模开始时，西药组灌胃盐酸氟西汀，中药组灌胃颐脑解郁复方，其余各组灌胃蒸馏水，每日1次。第2、4、8周末，各组大鼠进行旷场试验等行为学测试，HE染色观察海马CA1区病理变化。结果 除第2周外，同一时间点中药组大鼠各项行为学评分均高于PSD组。同一时间点中药组大鼠海马CA1区较PSD组结构完整，细胞排列整齐，形态正常。结论 颐脑解郁复方可改善大鼠PSD症状，并对海马CA1区具有保护作用。

关键词：颐脑解郁复方；卒中后抑郁；行为学；海马CA1区；大鼠

DOI：10.3969/j.issn.1005-5304.2017.07.012

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）07-0049-05

Abstract： Objective To investigate the intervention effects of Yinao Jieyu Prescription on the behaviors and damages in hippocampal CA1 area of the rats with post-stroke depression （PSD）. Methods Totally 168 SPF male SD rats were randomly divided into normal group， sham-operation group， stroke group， PSD group， Western medicine group and TCM group. There were 24 rats in the normal group and sham-operation group， and 30 rats in the other groups. Rats in the normal group received no intervention. Rats in the sham-operation group received no suture. Rats in the stroke group were given middle cerebral artery occlusion operation and normally fed after operation. Rats in the PSD group， Western medicinal group and TCM group were made into PSD models by chronic immobilization stress for one week and individual battery to the end. At the inception of modeling， Western medicine group received fluoxetine hydrochloride for gavage； TCM group received Yinao Jieyu Prescription for gavage； other groups received distilled water for gavage， once a day. At the end of week 2， 4， and 8， the morphology of the hippocampal CA1 area in each rat was observed by microscope after HE stained. Results Except for the week 2， at the same time point， the behavior scores of the rats in the TCM group were higher than those in the PSD group. At the same time point， the CA1 region of the hippocampus in the TCM group was more complete than the PSD group， and the cells were arranged neatly and in normal morphology. Conclusion Yinao Jieyu Prescription can improve the symptoms of PSD rats， and has protective effects on hippocampal CA1 area.

Key words： Yinao Jieyu Prescription； post-stroke depression； behaviors； hippocampal CA1 area； rats

卒中后抑郁（post-stroke depression，PSD）是臨床脑卒中后常见并发症，主要表现为情绪低落、思维迟滞、兴趣减退及社会角色的紊乱等。我国每年大约有160万～200万新发脑卒中患者，约1/3存活患者发生PSD[1-2]。颐脑解郁复方是本课题组自行研制治疗PSD的中药复方，具有益肾疏肝、活血化瘀的功效，前期临床研究显示疗效显著[3]。本实验建立一种高度模拟临床PSD的大鼠模型，以探讨颐脑解郁复方对PSD大鼠行为学及海马CA1区的干预作用。……