彭熙炜+阎卉芳+黄娟+朱嘉欢+徐昊+黄小平+邓常清

摘要：目的 觀察黄芪和当归不同比例配伍对大鼠血管内膜增生的影响，确立二者抗血管内膜增生的有效配伍。方法 采用基线等比设计方法将SD大鼠分为5个黄芪-当归不同配比组、单用黄芪组、单用当归组、阳性药组和假手术组，采用球囊导管损伤大鼠血管内皮造成胸腹主动脉血管内膜增生模型，同时灌胃黄芪、当归不同比例配伍药物，连续14 d，放血处死大鼠，取胸腹主动脉，行Masson染色，形态计量学方法分析血管内膜增生情况。结果 血管内膜损伤后14 d，胸腹主动脉内膜面积（IA）、内膜厚度（IT）、内膜面积增生率（HRIA）、内膜厚度增生率（HRIT）均增加。与模型组比较，单用当归组、芪归1∶2组、芪归1∶5组、芪归1∶1组、芪归5∶1组IA、IT、HRIA、HRIT均降低，且以芪归1∶1组作用最强。单用黄芪组、芪归2∶1组内膜增生各指标变化不明显。结论 黄芪和当归在一定比例范围配伍时具有抑制血管内膜增生的作用，其中以黄芪∶当归=1∶1配伍比例抗血管内膜增生的作用为佳。

关键词：黄芪；当归；血管内膜增生；配伍比例；大鼠

DOI：10.3969/j.issn.1005-5304.2017.06.014

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）06-0056-04

Abstract： Objective To observe the effects of different compatibility ratios of Astragali Radix and Angelicae Sinensis Radix on vascular intimal hyperplasia； To ascertain the effective compatibility of Astragali Radix and Angelicae Sinensis Radix for antagonizing vascular intimal hyperplasia. Methods SD rats were divided into different groups by baseline geometric design method： Astragali Radix and Angelicae Sinensis Radix different compatibility ratios groups， Astragali Radix group， Angelicae Sinensis Radixgroup， positive medicinegroup and sham-operation group. A model of intimal hyperplasia of thoracoabdominal aorta was established by balloon catheter injury in vascular endothelium of rats. Then the thoracoabdominal aorta was taken out after gavage of Astragali Radix and Angelicae Sinensis Radix with different compatibility ratios for 14 days. Bloodletting was used to take thoracoabdominal aorta. Masson staining and Morphometric methods were used to analyze the intimal hyperplasia. Results IA， IT， HRIA and HRIT increased 14 day after intimal injury. Compared with the model group， IA， IT， HRIA and HRIT in Angelicae Sinensis Radix group， Astragali Radix and Angelicae Sinensis Radix 1：2 group， Astragali Radix and Angelicae Sinensis Radix 1：5 group， Astragali Radix and Angelicae Sinensis Radix 1：1 group and Astragali Radix and Angelicae Sinensis Radix 5：1 group were lower， and the effects of Astragali Radix and Angelicae Sinensis Radix 1：1 ratio were strongest. The effects on intimal hyperplasia in Astragali Radix group and Astragali Radix and Angelicae Sinensis 2：1 group had no significant differences compared with model group. Conclusion Astragali Radix and Angelicae Sinensis can inhibit vascular intimal hyperplasia in a certain compatibility ratios， and the effects of Astragali Radix and Angelicae Sinensis Radix 1：1 on intimal hyperplasia are the best.

Key words： Astragali Radix； Angelicae Sinensis； vascular intimal hyperplasia； compatibility ratio； ratsendprint

基金项目：国家自然科学基金青年基金（81473851）；湖南省研究生科研创新项目（CX2016B378）；中西结合临床湖南省“十二五”重点开放基金（ZXLCXK201504）；湖南省科技创新团队（2013年）；湖南省自然科学创新群体基金（2013年）

通讯作者：邓常清，E-mail：dchangq@sohu.com

黄芪和当归是临床常用的中药药对，二者配伍既可补气生血，又能益气活血，具有药理多效性。研究表明，由黄芪和当归配伍组方具有促进造血[1]、心血管保护作用[2]、抗动脉粥样硬化[3]等功效。……