廖世业?李军俊?熊丽娟

[摘要] 目的 觀察联组运用特异性抗体检测试验ELISA法、胶体金法和非特异性抗体检测试验TRUST法检测诊断梅毒的效果。 方法 联组运用ELISA法、胶体金法和TRUST法对302例各期梅毒病例进行检测诊断，以其中两种及以上结果阳性判定为联组检测阳性，同时对全部样本用TPPA法作对照检测，计算四种检测方法各自对各期梅毒的阳性检出率。对联组检测试验组和对照试验组检测结果进行比较。 结果 56例潜伏期梅毒病例ELISA法、胶体金法、TRUST法和TPPA法阳性检出率分别为91.1%、94.6%、75.0%和96.4%；78例一期梅毒四种方法阳性率分别为97.4%、97.4%、96.2%和100.0%；102例二期梅毒四种方法阳性检出率均为100%；66例三期梅毒四种方法阳性率分别为100.0%、93.9%、66.7%和100.0%。联组检测试验组中ELISA法、胶体金法、TRUST法三种方法各自对潜伏期、一期和三期梅毒病例阳性检出率虽与对照组TPPA法稍有差别，但联组判别结果后56例潜伏期梅毒病例实际阳性54例，与对照组阳性率相等；78例一期、102例二期和66例三期梅毒，联组试验结果和对照组阳性检出率均为100%，二者对此三期梅毒检出结果比较差异无统计学意义（P>0.05）。 结论 联组运用特异性抗体检测试验ELISA法、胶体金法和非特异性抗体检测试验TRUST法检测不同期梅毒病例检出效果较好。

[关键词] 特异性；抗体；联合；检测；梅毒

[中图分类号] R759.1 [文献标识码] A [文章编号] 2095-0616（2017）05-19-04

[Abstract] Objective To observe the effect of specific antibody test ELISA method，colloidal gold method and non-specific antibody test TRUST method in joint detection of diagnosis of syphilis. Methods The joint of ELISA method，colloidal gold method and TRUST method was used to detect and diagnose the 302 cases of syphilis.In which two or more results were found for positive group.At the same time，all samples were detected by TPPA method.The positive detection rate of four methods for each stage of syphilis was calculated.The test results were compared between the test group and the control group. Results 56 cases of latent syphilis cases ELISA，colloidal gold，TRUST and TPPA positive detection rates were 91.1%，94.6%，75% and 96.4%，respectively.78 cases of primary syphilis of four methods positive rates were 97.4%，97.4%，96.2% and 100%，respectively.102 cases of secondary syphilis positive rate of the four methods were 100%.66 cases of the three stage syphilis positive rates of the four methods were 100%，93.9%，66.7% and 100%，respectively.There was slightly different on relevance ratio between ELISA method，colloidal gold method and TRUST joint test group in three methods of latency，one and three stage and TPPA of control group.But the results of 56 cases of latent syphilis cases group after the actual 54 cases of positive control group，and the positive rates were equal.For the 78 cases of primary syphilis，102 cases of secondary syphilis and 66 cases of three stage syphilis，the positive detection rates of joint test results and control group were 100%.There was no statistical difference between the two groups in the detection of syphilis in the three stage （P>0.05）. Conclusion The effect of specific antibody test ELISA method，colloidal gold method and non-specific antibody test TRUST method in joint detection of diagnosis of syphilis is good.

[Key words] Specificity；Antibody；Union；Syphilis；Detection

梅毒为乙类性传播传染病，近年发病率呈上升趋势。因较多梅毒病例临床表现呈现复杂性、不典型性的特点，临床诊断工作难度较大，从而使得血清学检测试验对梅毒的诊断，以及判定病程的发展、痊愈和药物疗效工作的意义更为突显[1]。为……