海水胁迫对金银花光合特性的影响
2017-05-19葛艳董祥开邵世光
葛艳+董祥开++邵世光



摘 要:为探究金银花的最适生长盐度,该文以连云港云台山风景管理处金银花为材料,研究不同盐度的海水(0、0.15%、0.3%、0.45%、0.6%)对其光合特性的影响。用Yaxin-1102便携式光合蒸腾仪隔天测量净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、细胞间CO2浓度(Ci)、气孔限制值(Ls)、水分利用率(WUE)。结果表明:低盐(0.15%)胁迫下,金银花气孔限制值下降,净光合速率、气孔导度、蒸腾速率、细胞间CO2浓度和水分利用率升高;中盐(0.3%)胁迫下,各指标与对照无显著差异;高盐(0.45%、0.6%)胁迫下,金银花净光合速率、气孔导度、蒸腾速率、水分利用率呈直线型下降,细胞间CO2浓度先降低后升高,气孔限制值先升高后降低。处理前期细胞间CO2浓度降低,气孔导度降低,说明净光合速率下降的主要原因是气孔因素,后期细胞间CO2浓度升高,气孔導度降低,说明此时净光合速率下降的主要原因转变为非气孔因素。在实验设计的5个盐度下,含盐量0.15%时,净光合速率最大。
关键词:金银花;海水胁迫;光合特性
中图分类号 S31 文献标识码 A 文章编号 1007-7731(2017)09-0035-05
Abstract:In order to explore the optimum growth salinity of honeysuckle,we choose the honeysuckle of Lianyungang Yuntai Mountain as the experimental materials. We design different salinity of seawater(0%,0.15%,0.3%,0.45%,0.6%)to explore effect on photosynthetic characteristics. We measure the netphotosynthetic rate(Pn),stomatal conductance(Gs),transpirati-on rate(Tr),intercellular CO2 concentration(Ci),stomatal limitation value(Ls),water use efficiency(WUE)by Yaxin-1102 portable photosynthesis. The results showed that:under low salt stress(0.15%),Pn, Gs, Tr,Ci and WUE was enhanced while stomatal limitation value was reduced;Under medium salt stress(0.3%),honeysuckle photosynthetic characteristics index had no significant difference with the control;Under the high salt stress(0.45%,0.6%),Pn,Gs,Tr and WUE was reduced. The photosynthesis of honeysuckle was inhibited. What is more,the greater the salinity,the more obvious inhibition. The main reason for the decline of photosynthetic rate in the early stage of treatment was stomatal factor. Later,it was changed into non stom-atal factors. It is concluded that the honeysuckle has a certain salt tolerance. Under the salt content of 0.15%,the value of net photosynthetic rate was max.
Key words:Honeysuckle;Seawater-stress;Photosynthetic
1引言
中国盐碱地分布广泛,土壤盐碱化是限制农业发展的问题之一[1]。连云港属沿海城市,全市滩涂地面积为5万hm2,占连云港土地总面积的6.7%,且该地海风不断,大气中也有一定的盐分[2];连云港土质偏碱性,土壤盐碱化较严重,因而该地域不适于多种植物的生长[3]。
金银花(Lonicera japonica),为忍冬科忍冬属植物,作为一种药用植物,它具有清热解毒的功效,经济价值高[4]。金银花对生长环境的要求并不严格,有着极强的适应力,喜温暖湿润的环境,最适生长温度为15~25℃、最适空气湿度为65%~75%、每日最适光照时长为7~8h、最适土壤含水量为10%。……
