杨晓云++宋涛++刘辉++王红

摘要：以玉米（Zea mays L.）幼苗為材料，通过水培试验，研究了不同浓度的外源甜菜碱对NaCl胁迫下玉米幼苗地上部、根系生长和叶绿素含量的影响。结果表明，在150 mmol/L NaCl胁迫下，不同浓度甜菜碱处理均能缓解NaCl胁迫对玉米幼苗的伤害，促进其生长，其中1.00 mmol/L甜菜碱处理的各项指标均优于其他浓度处理，与NaCl胁迫对照相比，其幼苗的株高、地上鲜重、根鲜重、地上干重和根干重分别提高了16.59%、14.35%、26.41%、32.63%和69.43%，叶绿素含量增加了24.27%，幼苗的总根长、总表面积、根体积、总分支数、总交叉数和根系活力分别提高了118.70%、71.38%、28.25%、176.15%、169.15%和63.99%，根系直径降低了30.25%。说明外源甜菜碱可通过促进幼苗的生长、增强根的吸收能力来缓解NaCl胁迫对玉米幼苗生长的抑制，且以1.00 mmol/L甜菜碱处理的缓解效果最好。

关键词：甜菜碱；NaCl胁迫；玉米（Zea mays L.）幼苗；根系生长；叶绿素含量

中图分类号：S513 文献标识码：A 文章编号：0439-8114（2017）05-0830-04

DOI：10.14088/j.cnki.issn0439-8114.2017.05.008

Effects of Exogenous Glycine Betaine on the Growth and Chlorophyll Content of Maize Seedlings under NaCl Stress

YANG Xiao-yun1，2， SONG Tao1， LIU Hui3， WANG Hong1

（1.Kingenta Ecological Engineering Group Co. Ltd.，Linshu 276700，Shandong，China；

2.State Key Laboratory of Nutrition Resources Integrated Utilization，Linshu 276700，Shandong，China；

3.Key Laboratory of Plant and New Fertilizer R & D，Ministry of Agriculture，P.R. China，Linshu 276700，Shandong，China）

Abstract： The experiment was carried out to study the effects of exogenous glycine betaine on the growth， root and chlorophyll content of maize（Zea mays L.） seedlings under NaCl stress in nutrient solution culture. The results showed that all growth indicators of maize seedlings were improved in different extent by supplying different concentrations of glycine betaine compared with 150 mmol/L NaCl treatment. The performance of all indicators under 1.00 mmol/L glycine betaine treatment were superior to the others. Compared with the NaCl stress treatment，its plant height，upper ground weight，root weight，shoot dry weight and root dry weight of an individual plant of seedlings was improved by 16.59%，14.35%，26.41%，32.63% and 69.43% respectively；chlorophyll content increased by 24.27%；total root length，（surface） area，volume，root total count，root system to the total number of cross and root activity was improved by 118.70%，71.38%，28.25%，176.15%，169.15% and 63.99% respectively； the root diameter was significantly reduced by 30.25%. The research illustrated that exogenous glycine betaine could improve the root activity and alleviate the inhibition of seeding growth under NaCl stress by promoting the growth of maize seedings. The mitigation effect of 1.00 mmol/L glycine betaine treatment was the best.

Key words： exogenous glycine betaine； NaCl stress； maize（Zea mays L.） seedlings； root growth； chlorophyll content

据统计，全世界有盐碱地10亿hm2，约占陆地总面积的1/3，中国约有667万hm2，与此同时，不适当的农业措施又造成了大量良田的次生盐渍化，使世界农业面临着极大挑战[1-3]。随着人口的增加和耕地面积的日益减少，开发利用盐碱地变得越来越重要[4]。玉米（Zea mays L.）是中国主要的粮食种植作物，是中国旱地作物种植面积最大的一种作物，其地位仅次于水稻，现居世界第二位[5]。由于玉米抵抗盐碱的能力较弱，当其受到外界环境的盐碱胁迫等逆境条件伤害时，经常会导致种子的不正常萌发进而导致玉米幼苗出苗不齐、不全、苗不健壮等一系列的现象，严重的时候还会影响到玉米的整体产量。……