APP下载

全自动血液分析仪外周血细胞计数的影响因素探讨

2016-10-24王宇

中国卫生产业 2016年26期
关键词:检测

王宇

中国民航飞行学院医院检验科,四川广汉618307

全自动血液分析仪外周血细胞计数的影响因素探讨

王宇

中国民航飞行学院医院检验科,四川广汉618307

目的探讨全自动血液分析仪外周血细胞计数的影响因素。方法选择中国民航飞行学院医院健康体检人群120例,在不同抗凝剂、不同温度和检测时间采用Sysmex XT-1800i全自动血细胞分析仪检测红细胞(RBC)、白细胞(WBC)和血小板(PLT)。结果肝素钠、枸橼酸钠和EDTA-K2抗凝剂检测WBC和RBC差异无统计学意义(P>0.05),肝素钠和枸橼酸钠抗凝的血标本PLT计数显著低于EDTA-K2抗凝血标本(P<0.05)。25℃室温保存下血标本WBC、RBC和PLT在30 min、2、4、6 h的测定结果差异无统计学意义(P>0.05)。在4℃低温保存下血标本WBC、RBC在30 min、2 h、4 h、6 h的测定结果差异无统计学意义(P>0.05)。而PLT在30 min、2 h、4 h、6 h的测定结果差异有统计学意义(P<0.05)。结论抗凝剂、温度和检测时间是影响外周血计数的重要因素,外周血细胞计数应该选择EDTA-K2抗凝剂、25℃、6h小时内完成检测。

外周血;细胞计数;影响因素

[Abstract]Objective To study the influence factors of peripheral blood cells counts of the fully automatic hematology analyzer.Methods 120 cases of healthy persons with physical examination in the Hospital of Civil Aviation Flight University of China were selected,and the red cells,white cells and blood platelets were tested by the Sysmex XT-1800i fully automatic hematology analyzer under different anticoagulant agents,temperatures and test time.Results The difference in detecting the white cells and red cells between the heparin sodium,sodium citrate and EDTA-K2 anticoagulant agent had no statistical significance(P>0.05),the blood platelet count of blood specimen of heparin sodium and sodium citrate anticoagulation was obviously lower than that of the EDTA-K2 anticoagulation blood specimen(P<0.05),the differences in the measurement results of the red cells,white cells and blood platelets of the blood specimens stored at a room temperature of 25℃after 30 min,2 h,4 h and 6 h(P>0.05),and the differences in the measurement results of blood platelet after 30 min,2 h,4 h and 6 h had statistical significance(P<0.05).Conclusion The anticoagulant agent,temperature and test time are the important influence factors of peripheral blood count,and the peripheral blood cell count should choose EDTA-K2 anticoagulant agent,and the test should be finished within 6 h at 25℃.

[Key words]Peripheral blood;Cell count;Influence factor

血液常规分析是临床诊断炎症、感染,以及血液系统疾病的重要辅助指标。血液常规分析组合项目包括外周全血细胞计数和白细胞分类计数、以及各指标衍生参数等。其中,红细胞、白细胞、血小板计数是血液常规分析的重要关键指标[1-2]。然而由于血液成分复杂多样,且存在个体差异性,影响外周静脉全血细胞计数的因素较多[3-5]。该研究从抗凝剂的类型、标本保存时间、保存温度3个方面对外周静脉全血细胞计数的影响进行探讨,以指导临床检验技术人员科学制定质量控制和保证措施,促进血液常规分析质量的提高,报道如下。……

登录APP查看全文

猜你喜欢

检测
QC 检测
小波变换在PCB缺陷检测中的应用