施　展　刘颖颖　武　荣扬州大学医学院附属淮安市妇幼保健院新生儿医学实验室，江苏淮安223002

不同剂量脂多糖对新生SD大鼠的影响

施展刘颖颖武荣▲
扬州大学医学院附属淮安市妇幼保健院新生儿医学实验室，江苏淮安223002

目的观察腹腔注射不同剂量脂多糖（LPS）对新生SD大鼠的影响。方法将42只新生SD大鼠按随机数字表法分为对照组（n=6）以及实验组（n=12），实验组分别为1 mg/kg组、2 mg/kg组、3 mg/kg组。实验组新生SD大鼠按照1、2、3 mg/kg剂量腹腔注射LPS；对照组给予等量生理盐水。观察24 h各组的一般情况、测定肺组织湿重/干重（W/D）比值，肺损伤病理评分，观察肺组织大体病理情况及肺组织病理学改变。结果注射LPS 24 h时，对照组肺结构完整、肺泡腔干净、肺泡隔无水肿和炎性细胞浸润，实验组病理切片可见炎症细胞浸润、肺泡壁和肺间质的水肿；各组24 h死亡数分别是0、0、0和4，4组死亡数比较，差异有统计学意义（χ2=7.174，P＜0.05）；实验组肺组织损伤评分［（1.91±0.10）、（2.80±0.11）、（5.28±0.39）分］、W/D比值［（1.77±0.07）、（2.98±0.26）、（4.77±0.21）］明显高于对照组［（0.71±0.80）分、（1.49±0.21）］，4组在肺组织损伤评分的两两比较，差异均有统计学意义（F= 699.761，P＜0.05）。4组在W/D比值的两两比较，除了对照组和1 mg/kg组比较差异无统计学意义（P＞0.05），其他各组差异均有统计学意义（F=587.936，P＜0.05）。结论给予新生SD大鼠腹腔注射不同剂量LPS（2 mg/kg和3 mg/kg）24 h内均可制作ALI模型。其中2 mg/kg诱导的大鼠肺损伤模型死亡率低，可以大量、快速地建模。

脂多糖；肺损伤；模型；新生鼠

［Abstract］Objective To observe the effect of newborn SD rats by intraperitoneal injection in different doses of lipopolysaccharide（LPS）.Methods 42 newborn Sprague-Dawley（SD）rats were divided into the control group（n=6）and the experiment groups（n=12）according to the random number table method.The experiment groups were divided 1 mg/kg group，2mg/kg group and 3mg/kg group；the experiment groups intraperitoneal injection of 1 mg/kg，2 mg/kg and 3 mg/kg LPS respectively；the control group were received the same volume saline injection.The general situation，wet weight/dry（W/D）weight ratio of lung，lung injury score and pathological changes of lung tissue at 24 hours in each group were observed.Results 24 hours after LPS injection，the control group lung structure was intact，alveolar cavity was clean，no edema and inflammatory cell infiltration were exist，the experiment group lung inflammatoty cell infiltration and interstitial edema were found；The mortality of groups at 24h were 0，0，0，4；There was significant differences in mortality between the control group and the experiment group（χ2=7.174，P＜0.05）；At experiment groups，the lung injury score［（1.91±0.10），（2.80±0.11），（5.28±0.39）］，the W/D ratios of lung tissue［（1.77±0.07），（2.98±0.26），（4.77± 0.21）］were significantly higher than those in the control group［（0.71±0.80），（1.49±0.21）］.There was significant differences in the lung injury score between the control group and the experiment groups（F=699.761，P＜0.05）.There was significant differences in the W/D ratios of lung tissue between the control group and the experiment groups（F= 587.936，P＜0.05），except for the control group and 1 mg/kg group（P＞0.05）.Conclusion It can be successfully established ALI model of newborn SD rats by intraperitoneal injection of different doses of LPS（2 mg/kg and 3 mg/kg）in 24h.2 mg/kg induced ALI model is with low mortality，it can be used to large and rapid establish ALI model.

［Key words］Lipopolysaccharide；Lung injury；Animal model；Newborn rat

急性肺损伤（acute lung injury，ALI）是各种致伤因素导致的肺泡上皮细胞及毛细血管内皮细胞损伤，造成弥漫性肺间质及肺泡水肿，导致急性低氧性呼吸功能不全，流行病学调查显示ALI是临床常见危重症，病死率较高［1］。……