陈达　廖州伟　马笃军　王立新　彭力平广州中医药大学深圳临床医学院广东省深圳市中医院，广东深圳518033

牛膝醇提物对兔骨关节炎模型的疗效比较

陈达廖州伟马笃军王立新彭力平▲
广州中医药大学深圳临床医学院广东省深圳市中医院，广东深圳518033

目的比较牛膝醇提物（AEAB）与硫酸氨基葡萄糖和双醋瑞因对于兔骨关节炎（OA）的疗效。方法将35只实验兔采用随机区组分组法分为正常组、模型组、AEAB组、硫酸氨基葡萄糖组和双醋瑞因组，每组7只。石膏固定制动6周制备OA模型，造模后，分别以蒸馏水、AEAB、硫酸氨基葡萄糖及双醋瑞因灌胃，正常组不予处理，6周后处死实验兔，取软骨标本进行大体观察、Pelletier评分、Mankin评分，用免疫组化测定软骨Ⅱ型胶原蛋白含量，用酶联免疫吸附测定（ELISA）法检测血清和关节液中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）以及基质金属蛋白酶-3（MMP-3）的含量。结果AEAB组、硫酸氨基葡萄糖组和双醋瑞因组Pelletier评分、Mankin评分、Ⅱ型胶原蛋白含量、血清和关节液中TNF-α、IL-1β以及MMP-3的含量分别与正常组、模型组比较，差异均有统计学意义（P＜0.05或P＜0.01）；AEAB组与硫酸氨基葡萄糖组及双醋瑞因组比较，上述指标差异均无统计学意义（均P＞0.05）。结论AEAB可以促进兔OA模型的软骨修复，其疗效与硫酸氨基葡萄糖及双醋瑞因相当，具有较高的临床应用价值。

牛膝醇提物；骨关节炎；硫酸氨基葡萄糖；双醋瑞因；疗效

［Abstract］Objective To compare the effects of alcohol extract of Achyranthis Bidentatae Radix（AEAB），glucosamine sulfate and Diacerein in the treatment of rabbits with osteoarthritis（OA）.Methods With blocked randomization，thirtyfive experimental rabbits were divided into normal group，model group，AEAB group，glucosamine sulfate group，Diacerein group，with 7 rabbits in each group.The OA models were established by immobilizing rabbits，knees with plaster cast for 6 weeks，and distilled water，AEAB，glucosamine sulfate，Diacerein were respectively given by lavage after modeling.Normal group was not treated.The experimental rabbits were killed six weeks later，and the cartilage samples were taken to perform gross observation，Pelletier score，Mankin score，and the typeⅡcollagen was measured by immunohistochemistry method and the tumor necrosis factor alpha（TNF-α），interleukin-1β（IL-1β）and matrix metalloproteinase-3（MMP-3）in serum and joint fluid were detected by enzyme-linked immunosorbent assay（ELISA）method. Results There were all statistically significant differences of Pelletier score，Mankin score，content of typeⅡcollagen，TNF-α，IL-1β and MMP-3 in AEAB group，glucosamine sulfate group，Diacerein group compared with normal group and model group respectively（P＜0.05 or P＜0.01），while there were no statistically significant differences in aforementioned indexes among AEAB group，glucosamine sulfate group and Diacerein group respectively（all P＞0.05）. Conclusion AEAB can promote the cartilage repair in rabbits with OA，and it has equivalent effects with glucosamine sulfate and Diacerein，so it has high value in clinical application.

［Key words］Alcohol extract of Achyranthis Bidentatae Radix；Osteoarthritis；Glucosamine sulfate；Diacerein；Effects

骨关节炎（OA）是以关节软骨退行性变为核心病理改变的慢性关节疾病，硫酸氨基葡萄糖和双醋瑞因作为治疗OA的西药代表，可以预防OA的进一步发展和促进软骨修复［1-2］。……