赵红伟　岳月红　康　薇.河北省人民医院消化内科，河北石家庄05005；.河北省人民医院神经内科，河北石家庄05005；.河北省新乐市医院消化内科，河北新乐050700

银杏叶提取物对肠炎小鼠肠黏膜通透性的影响

赵红伟1岳月红2康薇3
1.河北省人民医院消化内科，河北石家庄050051；2.河北省人民医院神经内科，河北石家庄050051；3.河北省新乐市医院消化内科，河北新乐050700

目的探索银杏叶提取物（EGB 761）对肠炎模型小鼠肠黏膜通透性的影响。方法30只C57BL/6小鼠随机分为正常组（10只）、模型组（10只）、EGB 761组（10只），建立急性结肠炎小鼠模型。观察小鼠一般情况及结肠病理变化；应用偶氮基质显色法测定血清中脂多糖（LPS）的含量；采用异硫氰酸荧光素（FITC-D）标记的右旋葡聚糖法以及细菌移位来检测肠黏膜通透性；免疫荧光染色及组织化学测定结肠组织中occludin、Claudin-1蛋白的变化。结果经EGB 761治疗后模型小鼠的一般情况及结肠组织病理学表现均有明显改善，EGB 761组小鼠血清中LPS的含量（0.14±0.04）与模型组（0.20±0.06）比较明显降低（P＜0.01）；EGB 761组FITC-D的渗透率与模型组（4200.80±326.60）比较明显降低（P＜0.01）；EGB 761组细菌移位率（40%）与模型组（80%）比较也明显降低（P＜0.01）。免疫荧光染色及组织化学染色结果显示，EGB 761组可增加Claudin-1、occludin蛋白阳性细胞染色。结论EGB 76可降低肠炎模型小鼠肠黏膜的通透性。

EGB 761；急性肠炎；动物模型；肠黏膜通透性

［Abstract］objective To investigate the effects of Gingko Biloba extract on the intestinal mucosal permeability in the colitis.Methods Thirty of C57BL/6 mice were randomly grouped as follows：control group（10 mice），model group（10 mice），EGB 761 group（10 mice）.Models of acute colitis were established，general condition and colon pathological changes were observed，the serum of LPS was detected by Azo matrix chromogenic method；intestinal mucosal permeability were detected by FITC-D and bacterial translocation，the expressions of occludin and Claudin-1 proteins were detected by immunofluorescence and immunohistochemistry.Results General condition and colon pathological were changed in the EGB 761 group compared with that of the model group；serum levels of the LPS in the EGB 761 group（0.14±0.04）was obviously decreased compared with those of the model group（0.20±0.06）（P＜0.01）；the level of the FITC-D in EGB 761 group was decreased obviously（1266.40±222.90）compared with that of the model group（4200.80±326.60）（P＜0.01）；Bacterial translocation was significantly decreased in the EGB 761 group（40%）compared with the model group（80%）（P＜0.01）；the results of immunofluorescence and immunohistochemical staining showed that the expressions of the Claudin-1 and occludin proteins in colonic tissues increased in EGB 761 group.Conclusion The administration of EGB 761 may be adjusted the intestinal mucosal permeability in the acute experimental colitis.

［Key words］EGB 761；Acute colitis；Animal model；Intestinal mucosal permeability

溃疡性结肠炎（Ulcerative colitis，UC）是一种慢性反复发作的肠道炎症性病变，目前多数人认为该病是在一定环境下使肠道黏膜屏障的作用削弱，肠黏膜的通透性增高，致使肠道的致病菌群容易穿过其屏障，从而导致炎症反复发生［1-5］，可见肠道黏膜通透性的增高与UC的发生有着紧密的联系［6-12］。……