陈　磊　俞雪锋　姚晓敏▲

1.浙江中医药大学药学院，浙江杭州310053；2.浙江医药高等专科学校药学院，浙江宁波315100

垂盆草不同极性部位体外抗氧化活性的研究

陈磊1,2俞雪锋2姚晓敏2▲

1.浙江中医药大学药学院，浙江杭州310053；2.浙江医药高等专科学校药学院，浙江宁波315100

目的研究垂盆草80%乙醇提取物不同极性部位的体外抗氧化活性。方法采用5种不同极性溶剂（石油醚、氯仿、乙酸乙酯、正丁醇、水）萃取得到5种萃取物，通过总还原能力、DPPH自由基清除能力、羟自由基清除能力和ABTS自由基清除能力4种方法对各萃取物的抗氧化能力进行研究。结果垂盆草不同极性部位提取物均具有一定的抗氧化活性，其中乙酸乙酯萃取部位的抗氧化能力最强，在总还原能力和清除DPPH自由基上，乙酸乙酯萃取部位作用明显强于其他四个极性部位，其清除ABTS自由基的半数浓度IC50为0.0131 mg/mL，清除羟基自由基的半数浓度IC50为1.0730 mg/mL。结论垂盆草醇提物的乙酸乙酯萃取部位具有较强的抗氧化活性，本研究为扩展垂盆草的应用范围和进一步研究其抗氧化活性成分提供了理论依据。

垂盆草；萃取物；抗氧化；自由基

［Abstract］Objective To study the in vivo antioxidant activity of parts with different polarity of the sedum sarmentosum 80%ethanol extract.Methods 5 extracts were obtained by 5 solvent with different polarity（petroleum ether，chloroform，ethyl acetate，n-butyl alcohol and water）and the antioxidant activity of each extract were studied by total reducing capacity，clearance capacity of DPPH free radicals，clearance capacity of hydroxyl radical and the clearance capacity of ABTS free radicals.Results Certain antioxidant activity was presented in the extracts of sedum sarmentosum parts with different polarity，and the activity of extract obtained by ethyl acetate was the strongest and was significantly stronger than the other four extracts in the respect of total reducing capacity and clearance capacity of DPPH free radicals.The IC50of clearing ABTS free radicals was 0.0131mg/mL and the IC50of clearing hydroxyl radicals was 1.0730 mg/mL. Conclusion Sedum sarmentosum extract obtained by ethyl acetate presents the strongest antioxidant activity and the study provide theoretical evidence for the extension of application range of sedum sarmentosum and further study of the antioxidant activity components.

［Key words］Sedum sarmentosum；Extract；Antioxidant；Free radical

人体在新陈代谢过程中会产生大量的自由基，正常情况下，自由基的生成与消除维持着一种动态平衡，但是当这种平衡被破坏时就会引起体内自由基的过量积累，从而对人体造成伤害，如肿瘤、心血管疾病等的发生均与自由基的过量积累及其诱导的系列氧化反应有着密切的联系［1，2］。目前，有研究表明中药的功效与其抗氧化作用有着紧密的联系，中药的黄酮、酚类、皂苷、多糖等物质均具有良好的抗氧化活性［3，4］，因此，研究中草药的抗氧化活性具有十分重要的意义。……