彭艳　雍翔智　冯豆豆　曾启新　梅国城　陶人川

1.广西医科大学附属口腔医院牙周黏膜科　南宁 530021；2.广西艾滋病防治研究重点实验室　南宁 530021

口腔慢性移植物抗宿主病患者唾液T细胞相关细胞因子的表达水平

彭艳1,2雍翔智1冯豆豆1,2曾启新1梅国城1陶人川1,2

1.广西医科大学附属口腔医院牙周黏膜科南宁 530021；2.广西艾滋病防治研究重点实验室南宁 530021

目的检测异基因造血干细胞移植后口腔慢性移植物抗宿主病（cGVHD）患者唾液T细胞相关细胞因子的表达水平，了解患者T细胞的免疫状态及其作用机制。方法移植组纳入确诊为口腔cGVHD患者18例，并对其口腔表征进行评分；对照组纳入健康者18例。收集两组唾液，采用流式液相多重蛋白定量技术检测T细胞相关细胞因子——干扰素（IFN）-γ、白细胞介素（IL）-4、IL-6、IL-10表达水平。结果移植组中IFN-γ，IL-4水平与对照组无统计学差异；移植组IL-6、IL-10表达量分别为255.04 pg·mL-1和1.49 pg·mL-1，显著高于对照组6.02 pg·mL-1和0.17 pg·mL-1，差异具有统计学意义（P＜0.01）；移植组中口腔表征评分与IL-6 、IL-10浓度均呈正相关关系，相关系数分别为r=0.978（P＜0.01）、r=0.668（P＜0.01），同时IL-6和IL-10浓度呈正相关关系，相关系数r=0.666 （P＜0.01）。结论口腔cGVHD患者唾液IL-6和IL-10表达呈上调趋势，口腔表征越严重，IL-6和IL-10表达水平越高，提示口腔cGVHD发病机制可能由Th2细胞相关细胞因子（IL-6、IL-10）介导。

造血干细胞移植；口腔慢性移植物抗宿主病；T细胞

This study was supported by the Natural Science Foundation of Guangxi（2014GXNSFAA118149）.

［Abstract］ObjectiveThis study aimed to explore the levels of T-cell cytokines in saliva of patients with oral chronic graft-versus-host-disease（cGVHD） who have undergone hematopoietic stem cell transplantation，as well as understand the T-cell immune status in patients. MethodsEighteen oral cGVHD patients were recruited as the transplantation group，and the scores of oral manifestations were recorded. Furthermore，18 healthy volunteers were selected as healthy controls. The unstimulated saliva of the two groups was collected，and the concentrations of T-cell cytokines such as interferon（IFN）-γ，interleukin（IL）-4，IL-6，and IL-10 in saliva were detected by cytometric bead array. ResultsIFN-γ and IL-4 levels in the transplantation group showed no difference from those of healthy controls. By contrast，the levels of IL-6 and IL-10 were significantly higher than those of healthy controls（255.04 pg·mL-1vs. 6.02 pg·mL-1and 1.49 pg·mL-1vs. 0.17 pg·mL-1，respectively；P＜0.01）. The scores of oral manifestations were positively related to IL-6（r=0.978，P＜0.01） and IL-10 （r=0.668，P＜0.01） levels. The levels of IL-6 and IL-10 were also positively correlated（r=0.666，P＜0.01）. ConclusionThe expression levels of IL-6 and IL-10 in saliva were both up-regulated in oral cGVHD patients. Severe oral manifestations corresponded to high levels of IL-6 and IL-10. Thus，Th2 cell cytokines（IL-6 andIL-10） might play a significant role in the pathogenesis of oral cGVHD.

［Key words］hematopoietic stem cell transplantation；oral chronic graft-versus-host-disease；T cell

异基因造血干细胞移植（hematopoietic stemcell transplantation，HSCT）是治疗血液系统恶性肿瘤的主要手段，其中慢性移植物抗宿主病（chronic graft-versus-host-disease，cGVHD）是常见的并发症。cGVHD被确认为是HSCT后，患者在无复发疾病影响的情况下高死亡率的原因及影响患者长期健康生存的重要因素［1-2］。……