卜小丽，刘　翼，刘世操，陈　帆，吴丰惟，韩朋伟，祝爱侠，2*（.武汉轻工大学动物营养与饲料安全湖北省协同创新中心，湖北武汉430023；2.武汉市畜禽饲料工程技术研究中心，湖北武汉430023）

植酸酶处理植物性蛋白质原料对吉富罗非鱼生长性能及磷利用率的影响

卜小丽1，刘翼1，刘世操1，陈帆1，吴丰惟1，韩朋伟1，祝爱侠1，2*
（1.武汉轻工大学动物营养与饲料安全湖北省协同创新中心，湖北武汉430023；2.武汉市畜禽饲料工程技术研究中心，湖北武汉430023）

为研究植酸酶体外预处理植物性蛋白质饲料原料对吉富罗非鱼生长性能、鱼体成分和磷利用率的影响，本试验选用500尾体重为（12.0±0.3）g的吉富罗非鱼，按试验要求随机分为5组，每组4个重复，每个重复25尾鱼，试验期为60 d。以豆粕、棉籽粕、菜籽粕等主要植物性蛋白质为原料设计配方，Ⅰ、Ⅱ、Ⅲ组分别在投喂植酸酶体外预处理植物性蛋白质饲料的基础上添加0％、0.6％、1.2％的Ca（H2PO4）2（MCP）；Ⅳ组为体内水解对照组、Ⅴ组作为空白对照组投喂未处理植物性蛋白质饲料和1.2％MCP，Ⅳ组在此基础上添加1280 U/kg植酸酶。结果表明：与空白组相比，植酸酶体外预处理植物性蛋白质饲料原料可以使吉富罗非鱼的增重率提高3.6％～9.7％，Ⅰ组粗灰分提高率高达32.8％，钙含量由1.58％提高到2.31％，粪磷降低在50％以上；与体内水解对照组相比，Ⅰ、Ⅱ、Ⅲ组总磷的储积量分别提高22.7％、22.2％、16.1％。由此可见，本试验条件下，水产饲料中添加适量的植酸酶体外预处理植物性蛋白质饲料原料可替代MCP的添加。

植酸酶；预处理；吉富罗非鱼；生长性能；钙磷沉积；磷利用率

［Abstract］This study was conducted to evaluate the effects of phytase in vitro pre-treated plant protein ingredients on growth performance，fish body composition and phosphorus accumulation rate of Yoshitomi tilapia.This project chose 500 Yoshitomi tilapia，which were randomly divided into 5 groupswith 4 replicates of 25 each for 60 d.The phytase-pretreated soybeanmeal，cottonmeal and rapeseedmeal as themain protein source were formulated.GroupⅠ，ⅡandⅢwere respectively added 0％，0.6％and 1.2％Ca（H2PO4）2based on phytase in vitro pretreatment plant protein diet，whereasⅣgroup added 1280 U/kg phytase on the basis ofⅣandⅤgroups feeds was included untreated feed and 1.2％MCP.The results showed that，compared with blank control group，the phytase in vitro pretreatment plant protein feed ingredients could make Yoshitomi tilapia weight gain rate increased 3.6％～9.7％.Crude ash increased rate of groupⅠwas up to 32.8％.Calcium content increased by 1.58％to 2.31％and fecal phosphorus decreased above 50％.Compared with the control group in vivo hydrolysis，the accumulation amount of total phosphorus of groupⅠ，Ⅱ，Ⅲwere increased by 22.7％，22.2％，16.1％.The results showed that under the experimental conditions，phytase in vitro pre-treatment plant protein feed in aquatic feed could substitude for MCP adding.

［Key words］phytase；pretreatment；Yoshitomi tilapia；growth performance；calcium and phosphorus deposition；phosphorus utilization

磷是水生动……