王琦，陈蔚清，张程

(贵州省人民医院妇科生殖中心，贵州 贵阳　550002)



不同激光辅助孵化对玻璃化冷冻和慢速程序冷冻卵裂期胚胎移植妊娠率的影响

王琦，陈蔚清，张程

(贵州省人民医院妇科生殖中心，贵州 贵阳550002)

目的：研究分析两种激光辅助孵化方法对玻璃化冷冻和慢速程序冷冻卵裂期胚胎移植妊娠率的影响。方法：对2013年11月到2014年7月期间在我院接受冷冻胚胎移植的160例患者进行临床研究，随机分为两组，对照组为进行激光透明带打孔辅助孵化，研究组进行激光透明带薄化辅助孵化，比较组间患者的临床妊娠结局。结果：胚胎经程序冷冻后,研究组胚胎种植率(19.1%)显著高于对照组(12.3%),差异有统计学意义(P<0.05),而解冻后卵裂球存活率(82.21% vs.77.43%)与临床妊娠率(37.5% vs.32.5%)无显著差异(P>0.05)；胚胎经玻璃化冷冻后，研究组和对照组解冻后卵裂球存活率(87.48% vs.87.41%)、胚胎的临床妊娠率(37.5% vs.35.0%)、种植率(17.5% vs.15.1%)相比较均无统计学差异(P>0.05)；研究组中来源于两种冷冻方法的卵裂期胚胎解冻后卵裂球存活率(87.48% vs.82.21%)、临床妊娠率(37.5% vs.37.5%)和种植率(17.5% vs.19.1%)无统计学差异(P>0.05)。结论：激光透明带削薄优于激光透明带打孔用于辅助孵化，不影响患者的妊娠率，可减少对胚胎的损害。

激光辅助孵化；冻胚移植；妊娠率

【Abstract】Objective：To study the influence of two kinds of laser assisted hatching method on frozen thawed embryo transfer pregnancy rate during cleavage stage by vitrification freezing and slow freezing program.Methods：In our hospital a clinical study was performed on 160 patients with frozen embryo used laser zona drilling assisted hatching,while the study group used laser zona pellucida thinning assisted hatching,clinical pregnancy outcome was compared between two groups.Results：Firstly,after cryopreservation,the embryo implantation rate in study group (19.1%)was significantly higher than that of the control group (12.3%),the difference was statistically significant (P<0.05),while the survival rate after thawing cleavage (82.21% vs.77.43%)and clinical pregnancy rate (37.5% vs.32.5%)had no significant difference (P>0.05);In addition,after vitrification freezing,the thawing cleavage survival rate (87.48% vs.87.41%),the clinical pregnancy rate of embryo (37.5% vs.35%)and implantation rate(17.5% vs.15.1%)had no statistically significant difference (P>0.05);What is more,the thawing cleavage survival rate (87.48% vs.82.21%),the clinical pregnancy rate of embryo (37.5% vs.37.5%)and the implantation rate (17.5% vs.19.1%)in the study group from two freezing methods,had no significant difference (P>0.05).Conclusion：The laser zona pellucida thinning assisted hatching is superior to laser zona drilling assisted hatching,it doesn't affect the patient’s pregnancy rate and can reduce the damage for the embryo.

【Key words】Laser assisted hatching;Frozen embryo transfer;Pregnancy rate

辅助孵化被用于辅助生殖相关技术已具有较长的时间，近几年随着显微激光相关医学的快速发展，激光系统由于具有快速、安全、准确等优点而被引入到辅助孵化，且已获得较好的临床效果[1-2]。本文关于两种激光辅助孵化方法对人类早期胚胎解冻后移植妊娠率的影响进行研究分析，所研究的结果报道如下。