陆会飞，牛小平*，沈浩，张伟（.皖南医学院弋矶山医院消化内科，安徽　芜湖400；.病理科）

自噬相关基因微管相关蛋白1轻链3和叉头框蛋白O1在胃癌组织中的表达及其意义

陆会飞1，牛小平1*，沈浩1，张伟2
（1.皖南医学院弋矶山医院消化内科，安徽芜湖241001；2.病理科）

目的：探讨自噬相关基因微管相关蛋白1轻链3（microtubule-associated protein 1 light 3，LC3）和叉头框蛋白O1（forkhead box protein O1，FoxO1）在胃癌组织中的表达及其意义。方法：应用免疫组织化学SP法检测29例胃癌组织和癌旁正常组织中自噬相关基因LC3和FoxO1的表达情况，分析自噬相关基因表达水平与临床病理特征之间的相关性。结果：免疫组化结果提示LC3蛋白、FoxO1蛋白在胃癌组织中的阳性表达率均高于癌旁正常组织（P＜0.01）。LC3蛋白表达水平与胃癌的分化程度、淋巴结转移、临床分期、侵及胃壁深度明显相关，与患者的性别、年龄、胃癌发生部位及大小无明显相关性；FoxO1蛋白表达水平与胃癌的分化程度明显相关，与患者的性别、年龄、胃癌发生部位、大小、淋巴结转移、临床分期、侵及胃壁深度无明显相关性。结论：自噬相关基因LC3和FoxO1在胃癌组织中表达水平增高，均与胃癌的分化程度相关，且FoxO1高表达是胃癌早期频发事件，提示自噬活动水平的上调对胃癌的发生、发展具有重要促进作用。

胃癌；自噬；微管相关蛋白1轻链3；叉头框蛋白O1

doi：10.16753/j.cnki.1008-2344.2016.04.005

［Abstract］Objective：To investigate the expression of autophagy related genes microtubule-associated protein 1 light 3（LC3）and forkhead box protein 1 light 3（FoxO1）in gastric cancer tissues and its significance.Methods：The expression of LC3 and FoxO1 protein were examined by immunohistochemical SP method in 29 gastric cancer tissues and adjacent cancer tissues，their relationship with some tumor pathological parameters was analyzed.Results：The positive rates of expression of LC3 and FoxO1 protein in gastric cancer tissues were significantly higher than those in adjacent cancer tissues（P＜0.01）.The expression level of LC3 in gastric cancer tissues was significantly correlated with tumor differentiation，lymph node metastasis，clinical stage and depth of tumor invasion，while it had no correlation with gender，age，tumor location and size of gastric cancer.The expression level of FoxO1 in gastric cancer was significantly correlated with tumor differentiation，while it had no correlation with gender，age，tumor location，size of gastric cancer，lymph node metastasis，clinical stage and depth of tumor invasion.Conclusion：Expression level of autophagy related genes LC3 and FoxO1 increased in gastric cancer tissues，and both of them are correlated with tumor differentiation，moreover，a high expression of FoxO1 is an frequent event in early gastric cancer，which suggested that increased level of autophagy activity plays an important role in promoting the occurrence and development of gastric cancer.

［Key words］gastric cancer；autophagy；LC3；FoxO1

自噬是一种复杂的降解代谢过程，多种自噬相关基因如微管相关蛋白1轻链3（microtubuleassociates protein 1 light 3，LC3）、叉头框蛋白O1 （forkheas box protein O1，FoxO1）和Beclin1等通过复杂而有序的调控来介导细胞自噬的发生［1］。无论正常的生理过程还是异常的病理过程，均有不同程度的自噬活动参与，肿瘤的发病过程亦不例外［2］。……