马涛+苏红燕

摘要：目的：从补充水解胶原蛋白（hydrolyzed collagen，HC）和跑台运动两个方面探索促进生长期骨生长的有效途径。方法：4周龄雄性SD大鼠48只，随机分为6组，每组8只：正常对照组（CON）、6%HC补充组（HC6）、12%HC补充组（HC12）、跑台运动组（CON+Ex）、6%HC补充+跑台运动组（HC6+Ex）和12%HC补充+跑台运动组（HC12+Ex）。跑台训练速度为30 m/min，每次训练60 min，每周训练6 d，共训练8 w。8w后测试各组大鼠股骨骨密度（bone mineral density，BMD）和形态学指标以及胫骨松质骨组织形态计量学指标。结果：与其相应的对照组相比，补充HC组与运动组大鼠股骨BMD和形态学指标，以及胫骨松质骨组织形态计量学指标均显著增加；高剂量补充HC组与中等剂量补充HC组相比，其上述指标差异无统计学意义；补充HC与跑台训练联合干预组与单纯补充HC组或单纯跑台训练组相比，上述指标均显著增加。结论：补充HC与跑台运动均能有效促进生长期的骨生长；中等剂量的HC补充即能较好地促进生长期的骨生长，而高剂量的补充并不能起到更好效果；补充HC与跑台运动具有协同作用，其效果优于单纯补充HC或单纯跑台运动。

关键词：水解胶原蛋白；跑台运动；骨密度；组织形态计量学

中图分类号：G804.4 文献标识码：A 文章编号：1006-2076（2016）04-0077-08

Abstract：Objective：This study aimed to explore effective ways to promote growth of growing bone from supplement hydrolyzed collagen and treadmill exercise. Methods：Forty-eight male SD rats， 4 weeks of age， were randomly divided into six groups and assigned as follows： normal control group （CON）， 6%HC supplemented group （HC6）， 12%HC supplemented group （HC12）， treadmill exercise group （CON+Ex）， 6%HC + supplement treadmill exercise group （HC6+Ex）and 12%HC + supplement treadmill exercise group （HC12+Ex）， each group of eight rats. Rats treadmill trained speed is 30m/min， eight weeks of training， training six days a week， 60 minutes a day of

training.After eight weeks， bone mineral density and morphological indicators of femur and histomorphometry indexes of tibia cancellous bone in each group were tested.Results：Compared with the corresponding control groups， bone mineral density and morphological indicators of femur and histomorphometry indexes of tibia cancellous bone in supplemented HC group and the exercise group were significantly increased. Compared with moderate-dose HC supplements group， the difference of these indexes in high-dose HC supplement group were not statistically significant. Compared with simply HC supplement group and simply treadmill training group， these indexes in combined HC supplement with treadmill training group were significantly increased.Conclusion：Supplementary HC and treadmill exercise both can effectively promote growth of growing bone. Moderate doses of HC supplement can better promote growth of growing bone， high-dose supplementation did not play better results. HC supplement with treadmill exercise have a synergistic effect， the effect is better than simply HC supplements and simply treadmill exercise.

Key words： hydrolyzed collagen； treadmill exercise； bone mineral density； histomorphometry

生长发育期是骨量增长的一个关键时期，此期间骨量的快速获得，是与骨骼本身处于突增生长阶段相吻合的。人生的最初20年里所获得的骨量可以达到其峰值骨量（PBM，Peak bone mass）的90%～99%，峰值骨量是维持成人生命的“骨库”，因而青少年时期骨量的获得对其成年后骨骼的健康起着决定性的作用[1]。峰值骨量的水平与成年后患骨质疏松症的可能性密切相关[2]。……