徐嘉辉 黄雪君 曾巧煌 蓝燕娇 张晓露 余德钊

摘要：目的 观察大桑菊合剂对小鼠迟发型变态反应性肝损伤的影响，探讨其抗炎免疫的作用机制。方法 采用腹腔注射环磷酰胺处理后，予2，4，6-三硝基氯苯2次外涂腹部皮肤致敏，再经皮肝穿刺攻击造模。实验小鼠随机分为空白组、模型组和中药高、中、低剂量组。中药高、中、低剂量组给予大桑菊合剂灌胃，每日1次，连续7 d。ELISA检测小鼠血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和肿瘤坏死因子-α（TNF-α）的水平，HE染色观察肝脏组织病理形态。结果 与正常组比较，模型组AST、ALT和TNF-α水平显著升高（P<0.01）；与模型组比较，中药各剂量组ALT、AST和TNF-α水平显著降低（P<0.05，P<0.01，P<0.001）；中药各剂量组较模型组均能减轻肝组织局部炎症浸润与水肿。结论 大桑菊合剂对小鼠迟发型变态反应性肝损伤具有保护作用。

关键词：大桑菊合剂；迟发型变态反应；肝损伤；小鼠

DOI：10.3969/j.issn.1005-5304.2016.07.019

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）07-0074-03

Protective Effects of Dasangju Mixture on Liver Injury Induced by Delayed-type Hypersensitivity in Mice XU Jia-hui1， 2， HUANG Xue-jun1， ZENG Qiao-huang1， LAN Yan-jiao1， ZHANG Xiao-lu1， YU De-zhao2 （1. The Second Provincial Hospital of Traditional Chinese Medicine， Guangzhou 510000， China； 2. Guangzhou University of Chinese Medicine， Guangzhou 510000， China）

Abstract： Objective To investigate the protective effects of Dasangju Mixture on the liver injury induced by delayed-type hypersensitivity in mice； To discuss its mechanism of anti-inflammatory-immunity. Methods After enterocoelia intravenous injection of cyclophosphamide was used， 2，4，6-picryl chloride was used twice for exterior coating for skin of abdomen to cause sensation of skin. Then percutaneous transhepatic puncture was used to attack for modeling. Male Kunming mice were randomly divided into blank group， model group， high-， medium- and low-dose treatment groups. The high-， medium- and low-dose treatment groups were given Dasangju Mixture for gavage， once a day for 7 days. ELISA was applied to determine the serum levels of aspartate aminotransferase （AST）， alamine aminotransferase （ALT） and tumor necrosis factor （TNF）-α. Pathological features of liver tissue were observed by HE staining. Results Compared with the blank group， the levels of AST， ALT and TNF-α in the model group increased significantly （P<0.05）； Compared with the model group， the levels of AST， ALT and TNF-α in the high-， medium- and low-dose treatment groups decreased significantly （P<0.05， P<0.01， P<0.001）； Compared with the model group， high-， medium- and low-dose treatment groups can better alleviate topical inflammation infiltration and edema of liver tissue. Conclusion Dasangju Mixture has certain protective effects on the liver injury induced by delayed-type hypersensitivity in mice.

Key words： Dasangju Mixture； delayed-type hypersensitivity； liver injury； mice

药物性肝损害是儿童常见的药物不良反应之一，世界卫生组织个例安全性病例报告数据库中报道，儿童药物肝损害占其他类型的损害1%，常见药物有扑

基金项目：广东省自然科学基金（2014A030313646）；广东省中医药局科技项目（20120108）

通讯作者：余德钊，E-mail：928279647@qq.com

热息痛、红霉素等[1]。肺炎支原体肺炎是儿童社区获得性肺炎常见病之一，其首选药物是大环内酯类药物，如阿奇霉素等[2]。然而，研究表明阿奇霉素序贯治疗小儿肺炎支原体肺炎与药物性肝炎有一定相关性，年龄越小相关性越大[3]。大桑菊合剂是我们的临床验方，其联合阿奇霉素治疗肺炎支原体肺炎能够明显缩短疗程，降低胃肠道及肝损害等不良反应发生率，且能双向调控促炎因子白细胞介素（IL）-6与抑炎因子IL-10，其作用机制与免疫调控相关[4]。……