高静

(辽宁中医药大学附属第四医院妇产科，沈阳　110101)

血管生成素-2及其受体在子宫内膜异位症中的表达及临床意义

高静

(辽宁中医药大学附属第四医院妇产科，沈阳110101)

【摘要】目的观察血管生成素-2(Ang-2)与酪氨酸激酶受体-2(Tie-2)在子宫内膜异位症(EMs)患者子宫内膜中的表达，探讨其在EMs的发生发展过程中的具体作用。方法选取本院2012年1月至2013年12月收治的70例EMs患者为研究组，选取同期70例非EMs患者为对照组，分别取材两组的内膜组织，应用免疫组织化学染色法测定Ang-2、Tie-2的表达状况。结果(1) Ang-2主要定位在细胞质中，主要表达于腺上皮细胞与血管内皮细胞，间质细胞中可见少量表达。Tie-2主要定位在腺上皮细胞胞浆中，少数定位在间质细胞与血管内皮细胞中。(2)研究组患者中Ang-2、Tie-2阳性表达率分别为75.7%和72.9%，显著高于对照组(分别为42.9%和38.6%)(P<0.05)。Ang-2在EMs患者增殖期的阳性表达率(67.5%)显著低于分泌期(86.7%)(P<0.05)；Tie-2在EMs患者增殖期的阳性表达率(87.5%)显著高于分泌期(53.3%)(P<0.05)。结论Ang-2、Tie-2在EMs患者内膜组织中均有较高表达，提示其参与EMs的发生发展。

【关键词】子宫内膜异位症；血管生成素-2；酪氨酸激酶受体-2；表达

Methods： Seventy patients with endometriosis (study group) and 70 patients without endometriosis (control group) were selected in our hospital from Jan 2012 to Dec. 2013. The endometrial tissues were collected for detecting the expression of Ang-2 and Tie-2 by immunohistochemical method.

Results： Ang-2 was mainly localized in the cytoplasm and expressed mainly in epithelial cells and vascular endothelial cells，and slightly expressed in stromal cells. Tie-2 is mainly located in the cytoplasm of epithelial cells，and a few located in the interstitial cells and vascular endothelial cells. The expression rate of Ang-2 and Tie-2 in the study group (75.7% and 72.9% respectively) were significantly higher than those in control group (42.9%，38.6%，respectively) (P<0.05). In the patients with endometriosis，Ang-2 expression rate in proliferative phase (67.5%) was significantly lower than that in secretory phase (86.7%)(P<0.05)，but Tie-2 expression rate in proliferative phase (87.5%) was significantly higher than that in the secretory phase (53.3%)(P<0.05).

Conclusions： Ang-2 and Tie-2 are highly expressed in endometrial tissues of the patients with endometriosis，which suggests that Ang-2 and Tie-2 may be involved in the development of endometriosis.

(JReprodMed2016，25(6)：545-549)

子宫内膜异位症(EMs)是临床中常见的妇科疾病，具有较高的发病率，临床表现主要为盆腔疼痛、不孕以及月经异常等，严重影响患者的健康和生活。EMs的发病机制目前尚不明确，但有研究证实EMs的发生发展与血管生成密切相关，异位内膜病灶中的微血管密度明显高于在位子宫内膜[1]。血管生成素-2(Ang-2)参与血管生成的过程，其通过与内皮细胞特异性酪氨酸激酶受体-2(Tie-2)的结合，促进血管的发生与生长[2]。本研究重点分析Ang-2与Tie-2在EMs患者异位内膜与在位内膜中的表达，并与非EMs患者正常子宫内膜中的表达情况进行比较，进一步了解二者在EMs发生发展过程中作用，以期为该病的诊断与治疗提供一定参考。……