柯毅湧 潘腾飞 叶如梦 张蒙 郭志雄 佘文琴 周玉 潘东明

摘 要 以‘中国水仙（Narcissus tazetta L. var. Chinensis Roem）盛花期花瓣为试验材料，采用高效液相色谱与多级质谱联用（HPLC-MSn）技术进行鉴定，比较采用2种提取方法（非酸水解法与酸水解法）提取类黄酮物质的优缺点。结果表明：用非酸水解法和酸水解法在盛花期花瓣中共鉴定到了4种黄酮醇类物质，1种酚酸类物质，其中，用非酸水解法首次在中国水仙花瓣中鉴定到了水仙苷，说明该法能在提取过程中避免水仙花瓣类黄酮物质糖苷键的断裂，获得更丰富的类黄酮物质结构信息，为中国水仙类黄酮物质成分的分析提供参考。

关键词 中国水仙；盛花期；酸水解法；非酸水解法；HPLC-MSn

中图分类号 S682.21 文献标识码 A

Abstract In this study，the petals of‘Narcissus tazetta L. var. chinensis Roemduring full-bloom stage were taken as the experimental materials. The comparison between non-acid hydrolyzation and acid hydrolyzation methods on flavonoids extraction was carried out. High Performance Liquid Chromatography（HPLC） and High Performance Liquid Chromatography-mass spectrometry（HPLC-MSn）techonlogy were employed to compare the advantages and disadvantages of the two extraction methods. The experiment results showed that four flavonols and one phenolic acid were identified in the petals during full-bloom stage by means of non-acid hydrolyzation and acid hydrolyzation. It was the first time that the narcissoside was identified in the petals of Narcissus by means of non-acid hydrolyzation，which indicating that this method could avoid the fracture of glycosidic bond of flavonoids in narcissus petals and obtain more abundant structural information. The results would provide references for analyzing the components of flavonoids in ‘Narcissus tazetta L. var. chinensis Roem.

Key words Narcissus tazetta L. var. chinensis Roem；Full-bloom stage；Acid hydrolyzation；Non-acid hydrolyzation；HPLC-MSn

doi 10.3969/j.issn.1000-2561.2016.08.027

花是观赏植物最主要的观赏部分，花色是观赏花卉最直观的观赏性状之一[1]，花色五彩缤纷，其主要由三大类花色素决定：即类黄酮、类胡萝卜素及与生物碱有关的其它色素，如甜菜碱、小檗碱等[2]。

中国水仙是石蒜科水仙属多花水仙（Narcissus tazetta）亚属的多年生草本植物，其花洁白清香，花婷玉立，绚丽多姿，素有“凌波仙子”的美称，是中国的十大传统名花之一[3]。中国水仙花色形成主要受类胡萝卜途径和类黄酮途径调控，其中类胡萝卜途径是中国水仙黄色副冠花色合成的主要途径[4]。张慧平[5]研究表明，中国水仙黄色副冠的花色素成分主要是类胡萝卜途径中的叶黄素和β-胡萝卜素，白色花瓣中2种色素含量都很少。在已有的报道中，关于中国水仙花瓣类黄酮途径的色素成分尚不明确。……