杨洋　马玲　　　马岩　　　陈旭日　潘佳亮　葛康康　姚翰文　刘雪峰

(东北林业大学，哈尔滨，150040)　(哈尔滨商业大学)　　　　　　　　　　　　　　　(东北林业大学)



α-蒎烯衍生物对山核桃干腐病菌作用机理1)

杨洋马玲马岩陈旭日潘佳亮葛康康姚翰文刘雪峰

(东北林业大学，哈尔滨，150040)(哈尔滨商业大学)(东北林业大学)

摘要为了探讨α-蒎烯衍生物3-甲氧基-4-羟基苯基-α-蒎烯马来酰亚胺基酰腙对病原菌的作用机理，采用平板扩散法研究了α-蒎烯衍生物对山核桃干腐病原菌(Botryosphaeria fusisporae)的抑制作用。结果表明：该衍生物可以抑制山核桃干腐病原菌生长，最大抑制率为63.37%，EC50为0.059 4 g·L-1，可导致山核桃干腐病菌菌丝体生长缓慢、质量减轻、菌液中pH值减小程度逐渐变大、电导率明显升高、大分子物质的吸光值增大、可溶性蛋白增加、过氧化物酶(POD)和过氧化氢酶(CAT)活性降低。说明α-蒎烯衍生物主要改变病原菌细胞膜的通透性，具有开发利用的潜力。

关键词α-蒎烯衍生物；作用机理；山核桃干腐病

分类号S763.1

α-pinene Derivative on Action Mechanism ofBotryosphaeriafusisporae//

Yang Yang, Ma Ling

(Northeast Forestry University, Harbin 150040, P. R. China); Ma Yan(Harbin University of Commerce); Chen Xuri, Pan Jialiang, Ge Kangkang, Yao Hanwen, Liu Xuefeng(Northeast Forestry University)//Journal of Northeast Forestry University，2016,44(5)：88-91,96.

To explore theα-pinene derivative of 3-methoxy-4-hydroxyphenyl-α-pinene maleimido acylhydrazone mechanism of pathogenic bacteria, we used the plate diffusion method to study theα-pinene derivative inhibition ofBotryosphaeriafusisporaepathogens. The derivative can inhibit the growth of B. fusisporae pathogens, the largest inhibition was 63.37%, EC50was 0.059 4 g/L, which led toB.fusisporaepathogens mycelial growth slow weight loss, pH value decrease degree of bacteria gradually increased, the electrical conductivity significantly increased, the absorbance macromolecules increases, the soluble protein increased, POD and CAT activity decreased. Theα-pinene derivative mainly changes the permeability of cell membrane pathogens with the potential development and utilization.

Keywordsα-pinene derivative; Function mechanism; Botryosphaeria fusisporae

山核桃干腐病是山核桃生产中发现的1种新病害，是一类主要由山核桃干腐病菌(Botryosphaeriafusisporae)引起的病害，又称溃疡病[1]。在山核桃主栽培区普遍发生，个别地区受害严重，病株上少则几个病斑，多则几十个病斑，最严重的可达数百个病斑，发病率在30%以上，不但影响山核桃的生长和产量，而且严重时会导致树木过早死亡，造成重大经济损失[2]。目前，关于该病的防治方法研究报道较少，只见物理刮除病斑法[3]和化学农药喷洒法[4]。直接刮除病斑会造成树木产生新的伤口，易再度引发病菌侵入，且防治效果较短；……