侯文娟　胡厚臻　鲁莹莹　李桃祯　滕维超　王凌晖

(广西大学，南宁，530000)



磷对铝胁迫下尾巨桉生长生理方面的缓解作用1)

侯文娟胡厚臻鲁莹莹李桃祯滕维超王凌晖

(广西大学，南宁，530000)

摘要实验以尾巨桉Dp2-29(E. urophylla×E. grandis Dp2-29)为实验材料，用沙培法研究外源磷(P)对铝胁迫下尾巨桉生长和生理方面的缓解作用。结果表明：(1)5 mmol·L-1铝显著抑制尾巨桉的根、茎、叶生物量，显著提高根冠比。(2)铝胁迫抑制叶片叶绿素a、叶绿素b和类胡萝卜素质量分数，降低ω(叶绿素a)/ω(叶绿素b)值。(3)铝胁迫下叶片细胞膜透性显著增大，膜脂过氧化的产物丙二醛显著增加，细胞可溶性糖和可溶性蛋白质量分数显著降低，游离脯氨酸质量分数显著增大。(4)P缓解铝胁迫对光合色素的影响以及调节活性氧代谢以维持细胞膜结构稳定，有效缓解铝胁迫对尾巨桉生长、光合色素以及抗性生理造成的伤害，磷铝互作有利于缓解铝胁迫，P施用浓度为200 μmol·L-1时，缓解铝胁迫效果较好。

关键词尾巨桉Dp2-29；铝胁迫；磷；生长；生理

分类号Q945

Relieve Effect of Exogenous P on the Eucalyptus Growth and Physiological Feature under Al Stress//

Hou Wenjuan, Hu Houzhen, Lu Yingying, Li Taozhen, Teng Weichao, Wang Linghui

(Guangxi University, Nanning 530000, P. R. China)//Journal of Northeast Forestry University，2016,44(5)：5-9，19.

To verify the growth and physiological Feature of exogenous phosphorus on ameliorating aluminum toxicity in Eucalyptus, we plantedE.urophylla×E.grandisDp2-29 in sand solutions, and then detected the root and above-ground biomass, photosynthetic pigments, and antioxidant physiological indexes. Al of 5 mmol·L-1significantly inhibited the root and above-ground biomass and increased the root shoot ratio. Al of 5 mmol·L-1significantly inhibited the leaf Chl a/b and the contents of leaf Chl a, Chl b and Car. Al stress significantly increased cell membrane permeability and MDA (the product of plasmalemma peroxidation) and free proline contents, decreased soluble sugar and soluble protein content inEucalyptusleaf. Exogenous P played an important role in formation and inhibit decomposition of photosynthetic pigments, maintaining cell membrane structure stability by regulating reactive oxygen metabolism under Al toxicity, thereby reducing the Al damage to Eucalyptus growth photosynthetic pigments and resistance physiology. P of 200 μmol·L-1was better in the relieve effect under Al stress.

KeywordsEucalyptus urophylla×E. grandis Dp2-29; Al stress; P; Growth; Physiological

铝是地壳中含量最丰富的金属元素，一般情况下都以难溶性的化合物形式存在于土壤中，对植物体没有伤害，但当土壤为酸性，特别是pH低于5时，铝被大量活化为活性羟基铝和3价铝(Al3+)等土壤高活性铝，Al3+对植物生长的影响最大，是导致植物铝中毒最主要的原因[1-2]。据了解，全球酸性土壤占可耕作土地的30%左右[3]。另外，由于酸雨的大面积发生和连续施用含氨和氨化物的肥料等导致酸性土壤面积不断增大，现在研究普遍认为铝胁迫已成为酸性土壤中致使森林衰退和农作物减产的最主要的原因之一[4-6]。……