陈业雯+刘芳+高玉刚+汪志强+李雨琦+谢守峰+陈学武+杨瑾

摘 要：该文主要研究了休眠的细叶百合鳞茎在低温5℃贮藏过程中，抗氧化酶及呼吸代谢与鳞茎休眠进程的关系。结果表明，鳞茎呼吸速率在冷藏2周后显著下降，2～12周随着贮藏时间的延长呼吸速率逐渐上升。SOD活性在冷藏2～6周呈下降趋势，6～12周后逐渐增加。CAT活性4周最高，POD 活性在贮藏过程中逐渐降低。鳞茎ASP活性则表现为先升高后降低的趋势，PPO活性在贮藏初期平缓下降。4周后逐渐上升，整个冷藏期PAL活性平稳波动。鳞茎贮藏过程中呼吸速率与SOD、POD、PAL呈正相关性，呼吸速率与CAT、ASP、PPO均表现为负相关性。

关键词：细叶百合；鳞茎；低温贮藏；抗氧化酶

中图分类号 S68 文献标识码 A 文章编号 1007-7731（2016）06-15-03

Effects of Antioxidant Enzymes on Bulb Dormancy-broken in Lilium pumilum

Chen Yewen1 et al.

（1College of Agriculture，Heilongjiang Bayi Agricultural University，Daqing 163319，China）

Abstract：The changes of respiratory rate and antioxidant enzymes activity of Lilium pumilum during bulb dormancy breaking at 5℃ were studied. Results showed that the respiration rate decreased observably at 2w，respiration rate increased with the extension of cold storage time within 2～12w，SOD activity of scales was falling within 2～6w，and on the rise in 6～12w. The maximum value of CAT activity rose at 4w，POD activity had a downward trend during cold storage，ASP activity rose first and falled later，PPO activity had a trend of gentle decline during early storage，increased after 4w，PAL activity changed smoothly in the whole cold storage period. Respiratory rate and SOD，POD，PAL were characterized by positive correlations. Respiratory rate and CAT，ASP，PPO were in the form of negative correlation.

Key words：Lilium pumilum；Bulb；Low temperature storage；Antioxidant enzymes

百合是重要的园林应用花卉，其具有良好的食用、药用、观赏价值。百合鳞茎具有秋季收获后休眠、春季复苏破眠的特性，已有很多学者从鳞茎内部细胞组织结构、核酸、碳水化合物及相关酶的生理生化代谢方面对其进行了系统的研究；超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）等抗氧化酶类在百合鳞茎休眠解除过程中也具有重要的作用。乔永旭等[1]指出抗坏血酸过氧化物酶（ASP）和POD的活性在百合鳞茎低温解除休眠过程中先上升后下降。孔红梅等研究表明，随着贮藏期的延长，鳞茎盘PAL活性下降，顶芽与鳞片的 PAL活性有增加趋势[2]。植物破眠过程中，多种活性氧代谢相关基因进行表达[3-4]，H2O2在活化戊糖磷酸途径中起重要作用[5]。因此，冷藏过程中活性氧变化与抗氧化酶代谢可能影响百合鳞茎休眠。……