刘海卿，武军艳，孙万仓，刘自刚，钱武，杨建胜，马骊，方彦，李学才

(甘肃省作物遗传改良与种质创新重点实验室，甘肃省油菜工程技术研究中心，甘肃省干旱生境作物重点实验室，甘肃 兰州 730070)

不同叶龄期叶面喷施ABA对北方白菜型冬油菜抗寒性的影响

刘海卿，武军艳，孙万仓*，刘自刚，钱武，杨建胜，马骊，方彦，李学才

(甘肃省作物遗传改良与种质创新重点实验室，甘肃省油菜工程技术研究中心，甘肃省干旱生境作物重点实验室，甘肃 兰州 730070)

摘要：为了研究ABA对北方白菜型冬油菜抗寒性的影响,本试验以白菜型冬油菜陇油8号为材料，分别于冬油菜冬前三、五、六、七叶期用10 mg/L的脱落酸(ABA)溶液喷施叶片，以各个时期喷蒸馏水为对照，分析其对越冬率、产量及生理生化指标的影响。结果表明，外源ABA喷施能够显著提高白菜型冬油菜的越冬率和产量，提高保护性酶活性及渗透调节物等生理生化物质含量，且这种作用主要发生在受到低温(-5℃)胁迫以后， ABA处理的越冬率和产量分别较CK增加14.3%, 8.0%；受低温胁迫后的SOD、POD、CAT酶的活性分别较CK提高39.8%, 20.8%，31.0%；SP、SS、Pro含量较CK增加104.5%，120.1%，77.7%，且随喷施时期总体呈先升高后降低的趋势，六叶期喷施效果最佳。同时ABA处理能增强根系活力，降低MDA含量。因此，在北方寒旱区，在白菜型冬油菜冬前六叶期喷施一定浓度的ABA能提高冬油菜的抗寒性。

关键词：白菜型冬油菜；ABA；时期；抗寒性。

Effect of exogenous abscisic acid on the cold resistance ofBrassicarapacv. Longyou (winter rape) 8 cultivated at different foliar in northern China

LIU Hai-Qing, WU Jun-Yan, SUN Wan-Cang*, LIU Zi-Gang, QIAN Wu, YANG Jian-Sheng, MA Li, FANG Yan, LI Xue-Cai

ImprovementandKeyLaboratoryofCropGeneticsandGermplasmEnhancement,RapeseedEngineeringResearchCenterofGansuProvince,GansuProvincialKeyLaboratoryofAridLandCropSciences,GansuProvince,Lanzhou730070,China

Abstract:The aim of this study was to determine the effects of exogenous abscisic acid (ABA) on the cold resistance of Brassica rapa (winter rape, cultivar Longyou 8) under low temperature stress. Leaves of Longyou 8 were sprayed with 10 mg/L ABA when the plants were at the three-, five-, six-, and seven-leaf stages before winter. Plants at the same stages were sprayed with distilled water as the control. The overwintering rate, yield and physiological and biochemical indexes of Longyou 8 were determined after a cold treatment (-5℃). The plants sprayed with ABA showed significantly increased overwintering rates and yields, higher activities of antioxidant enzymes, and increased contents of physiological and biochemical substances that function as osmotic regulators. The effect was extremely significant after low temperature stress, when the overwintering rate and yield of ABA-treated plants were 14.3% and 8.0% higher than their respective values in the control plants. The activities of superoxide dismutase, peroxidase, and catalase were 39.8%, 20.8%, 31.0% higher, respectively, in ABA-treated plants than in control plants after the low temperature treatment. The contents of soluble protein, soluble saccharides, and free proline were 104.5%, 120.1%, and 77.7% higher, respectively, in the ABA-treated plants than in control plants after the low temperature treatment. The best stage for ABA application was the six-leaf stage. Application of ABA also increased root activity and decreased the contents of malondialdehyde, a marker of lipid peroxidation. Together, these results show that spraying with ABA can improve the cold resistance of winter rape in northern China.

Key words:Brassica rape winter rape (Brassica campestris); ABA; stage; cold resistance

由于超强抗寒冬油菜(Brassicacampestris)品种的选育和冬油菜北移示范成功，我国北方寒旱区冬油菜的种植面积逐渐扩大[1]，但该地区冬春季寒冷干燥，低温冻害成为影响该地区冬油菜生产的主要限制因素之一。……